FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82705
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC16451.9889970376639865No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14711.7786107248654857No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11741.419503053019769No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11011.3312375309836164No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10701.293754912036757No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG10511.2707816939725531No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10161.2284626080648087No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG9251.1184329847046732No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8260.9987304274227676No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7890.9539931080345808No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7700.9310198899703767No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG7300.882655220361526No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG6680.8076899824678072No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6680.8076899824678072No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5800.7012877093283356No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5430.6565503899401488No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4660.5634484009431111No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4500.5441025330995708No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4390.5308022489571368No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4190.5066199141527115No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4070.4921105132700563No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4060.49090139652983494No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3810.4606734780243033No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3780.45704612780363946No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3370.40747234145456745No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3170.38329000665014207No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3150.3808717731696995No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3120.37724442294903576No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2950.35668943836527417No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2830.34218003748261894No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2740.33129798682062755No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2700.3264615198597425No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2660.3216250528988574No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2410.2913971343933257No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2350.28414243395199806No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2300.2780968502508917No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2250.2720512665497854No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2200.26600568284867904No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2180.2635874493682365No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2120.2563327489269089No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2110.2551236321866876No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2040.24665981500513876No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2030.24545069826491747No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1990.24061423130403242No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1840.2224774802007134No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1770.2140136630191645No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1600.19345867843540293No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1560.18862221147451788No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1560.18862221147451788No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1550.1874130947342966No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1540.18620397799407531No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1480.17894927755274773No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1450.1753219273320839No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1450.1753219273320839No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1360.1644398766700925No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1350.16323075992987124No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1240.1499304757874373No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1240.1499304757874373No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1210.14630312556677347No Hit
GACTACTCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1170.1414666586058884No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1170.1414666586058884No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1110.1342119581645608No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1080.13058460794389698No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1070.12937549120367572No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1040.1257481409830119No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1030.12453902424279063No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1030.12453902424279063No Hit
GACTACTGGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1020.12332990750256938No Hit
GACTACTCGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC980.1184934405416843No Hit
GACTACTGGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC970.11728432380146303No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG950.11486609032102049No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA950.11486609032102049No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC910.11002962336013541No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA910.11002962336013541No Hit
GACTACTAGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC900.10882050661991415No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACACTTTCGCGCCTCAC900.10882050661991415No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG890.10761138987969289No Hit
GACTACCCGGGTATCTAATCCGAAGACCTCCTTCCTCCACGCGGCGTCGC880.10640227313947162No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT880.10640227313947162No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG870.10519315639925034No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC850.10277492291880781No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG840.10156580617858654No Hit
GACTACCAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA830.10035668943836526No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACATATCTGACGCTGCCGACGA830.10035668943836526No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)