FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008119501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78803
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT24493.107749704960471No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16062.0379934774056823No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14181.7994238798015303No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13991.7753131225968555No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT12031.526591627222314No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10311.3083258251589407No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8321.055797368120503No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5960.7563163838940141No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4980.6319556362067433No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3660.4644493229953174No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT3530.44795248911843455No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3430.4352626169054478No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3090.3921170513812926No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3010.38196515361090316No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2980.37815819194700706No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2710.3438955369719427No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2540.3223227542098651No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2520.3197847797672677No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2330.2956740225625928No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2320.2944050353412941No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG2240.28425313757090465No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2160.2741012398005152No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2160.2741012398005152No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC2100.2664873164727231No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2090.2652183292514244No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2090.2652183292514244No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2020.2563354187023337No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2020.2563354187023337No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1990.2525284570384376No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1940.24618352093194423No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1880.2385695976041521No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1840.2334936487189574No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1840.2334936487189574No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1760.22334175094856795No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1760.22334175094856795No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1740.2208037765059706No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1720.21826580206337323No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1610.20430694262908775No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1580.20049998096519167No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1560.19796200652259432No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1530.19415504485869828No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1510.1916170704161009No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1510.1916170704161009No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1510.1916170704161009No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1500.19034808319480223No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1480.18781010875220489No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1450.1840031470883088No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1430.18146517264571146No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1420.1801961854244128No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1400.17765821098181542No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1380.17512023653921807No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1360.1725822620966207No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT1360.1725822620966207No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1340.17004428765402332No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1330.16877530043272465No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1330.16877530043272465No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT1320.16750631321142598No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1310.16623732599012728No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGAAAACCCTGAT1310.16623732599012728No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1290.1636993515475299No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1280.16243036432623123No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTTGCTGGACTGTAACTGACG1280.16243036432623123No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1250.15862340266233518No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC1240.1573544154410365No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTATACATAGCGGAT1230.15608542821973784No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1190.15100947933454312No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACGATGGGGGAAACCCTGAT1190.15100947933454312No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1170.14847150489194574No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1150.14593353044934837No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1150.14593353044934837No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT1140.1446645432280497No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1140.1446645432280497No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1140.1446645432280497No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGGTCAATGGGGGAAACCCTGAA1140.1446645432280497No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1130.14339555600675102No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1130.14339555600675102No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAC1120.14212656878545232No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTATACATAGCGGAT1090.1383196071215563No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1070.13578163267895893No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG1040.13197467101506288No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC1000.12689872212986814No Hit
CCTACGGGGGGCAGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1000.12689872212986814No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT990.1256297349085695No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG980.12436074768727079No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT980.12436074768727079No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTTTCTGGACGACAACTGACG980.12436074768727079No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT970.12309176046597212No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG970.12309176046597212No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTATACATAGCGGAT960.12182277324467343No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAGACCCTGAT950.12055378602337476No Hit
CCTACGGGTGGCAGCAGTCGGTCGGGCAGCGTCAGATGTGTTGAACCTGG950.12055378602337476No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC940.11928479880207606No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT940.11928479880207606No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT920.1167468243594787No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT910.11547783713818002No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTACACAATGGGGGAAACCCTGAT910.11547783713818002No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC910.11547783713818002No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC900.11420884991688134No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGCTTGCTGGACTGTAACTGACG890.11293986269558266No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAGCCCTGAT880.11167087547428398No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACGATGGGGGAAACCCTGAT880.11167087547428398No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGCTTGCTGGACTGTAACTGACG880.11167087547428398No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGAAAACCCTGAT880.11167087547428398No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT870.1104018882529853No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT860.10913290103168662No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT860.10913290103168662No Hit
CCTACGGGTGGCTGCAGTGGTGAATATTACACAATGGGGGAAACCCTGAT850.10786391381038792No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTATACATAGCGGAT850.10786391381038792No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT850.10786391381038792No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT840.10659492658908924No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC830.10532593936779056No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGCTTGCTGGACTGTAACTGACG830.10532593936779056No Hit
CCTACGGGTGGCTGCAGGAGAGGAAAACCTGGCGAGAGATGGTTGAGAGA830.10532593936779056No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCAGGAGGAACACCAGTGGCGA820.10405695214649188No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC820.10405695214649188No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT820.10405695214649188No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC810.1027879649251932No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGAAAACCCTGAT800.10151897770389452No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT800.10151897770389452No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT790.10024999048259584No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC790.10024999048259584No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTATACATAGCGGAT790.10024999048259584No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)