FastQCFastQC Report
Wed 8 May 2024
EGAF00008119512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94435
Total Bases28.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG81018.578387250489754No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG70617.477100651241594No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG59956.348281887012231No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG56746.0083655424365965No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG47835.064859427119182No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG45664.835071742468364No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG43194.573516175146927No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG37063.924392439243925No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG33003.4944670937682005No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5930.6279451474559221No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5520.5845290411394081No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4160.4405146396992641No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3910.41404140414041407No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3600.38121459204744007No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3430.363212791867422No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3180.33673955630857205No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2910.30814846190501405No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2700.28591094403558004No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2630.278498438079102No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2470.26155556732143803No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2420.256260920209668No Hit
GACTACTGGGGTATCTAATCNTGTTCGCTACCCATGCTTTCGCTCCTCAG2400.25414306136496No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2370.25096627309789804No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2240.237200190607296No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2220.23508233176258803No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2100.22237517869434No Hit
GACTACTCGGGTATCTAATCNTGTTCGCTACCCATGCTTTCGCTCCTCAG2080.22025731984963204No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGNTTTCGCTCCTCAG2060.21813946100492404No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1920.203314449091968No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1890.200137660824906No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1820.192725154868428No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGNTTTCGCTCCTCAG1810.19166622544607403No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1810.19166622544607403No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1780.188489437179012No Hit
GACTACCGGGGTATCTAATCNTGTTCGCTACCCATGCTTTCGCTCCTCAG1740.184253719489596No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1710.18107693122253402No Hit
GACTACTAGGGTATCTAATCNTGTTCGCTACCCATGCTTTCGCTCCTCAG1690.17895907237782602No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1670.17684121353311802No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1670.17684121353311802No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1660.17578228411076402No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGNTTTCGCTCCTCAG1590.16836977815428603No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1570.166251919309578No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG1550.16413406046487No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGNTTTCGCTCCTCAG1490.15778048393074603No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1490.15778048393074603No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1460.15460369566368404No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1450.15354476624133No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1420.150367977974268No Hit
GACTACAGGGGTATCTAATCNTGTTCGCTACCCATGCTTTCGCTCCTCAG1400.14825011912956002No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG1390.14719118970720602No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1380.14613226028485202No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1370.14507333086249802No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1360.14401440144014402No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1350.14295547201779002No Hit
GACTACCAGGGTATCTAATCNTGTTCGCTACCCATGCTTTCGCTCCTCAG1320.13977868375072802No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG1290.13660189548366602No Hit
GACTACCCGGGTATCTAATCNTGTTCGCTACCCATGCTTTCGCTCCTCAG1280.13554296606131203No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1280.13554296606131203No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1270.134484036638958No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGNTTTCGCTCCTCAG1250.13236617779425003No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGNTTTCGCTCCTCAG1200.12707153068248No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGNTTTCGCTCCTCAG1200.12707153068248No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1180.124953671837772No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1180.124953671837772No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG1110.11754116588129401No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1080.114364377614232No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGCTCCTCAG1050.11118758934717No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1050.11118758934717No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1040.11012865992481602No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1020.10801080108010801No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1000.10589294223540002No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG980.10377508339069201No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG970.10271615396833803No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG960.101657224545984No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG950.10059829512363001No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG950.10059829512363001No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)