FastQCFastQC Report
Wed 8 May 2024
EGAF00008119519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83913
Total Bases25.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT21022.5049753911789594No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14111.6815034619188922No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT13131.5647158366403298No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11491.369275320868042No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11231.3382908488553622No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9311.1094824401463421No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7240.8627983745069299No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6580.7841454840132042No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT5370.6399485181080405No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5350.6375650971839882No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT5240.6244562821017006No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4770.5684458903864718No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3980.4743007638864062No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3790.4516582651079094No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3780.45046655464588325No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3600.42901576632941263No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3570.4254406349433341No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3550.42305721401928187No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3520.4194820826332034No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3450.4111401093990204No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3300.39326445246862823No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3060.36466340138000075No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3040.3622799804559484No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3020.35989655953189614No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2990.3563214281458177No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2860.3408291921394778No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2770.3301037979812425No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2700.32176182474705944No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2690.3205701142850333No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2640.3146115619749026No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2540.3026944573546411No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2540.3026944573546411No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2480.29554419458248427No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2430.2895856422723535No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2350.28005195857614434No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2350.28005195857614434No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2340.2788602481141182No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2230.2657514330318306No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1940.23119182963307233No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1880.22404156686091545No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1860.22165814593686317No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1850.22046643547483702No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1840.2192747250128109No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1810.21569959362673247No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1770.21093275177862786No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1730.20616590993052328No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1670.1990156471583664No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1620.1930570948482357No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1580.1882902530001311No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1530.18233170069000038No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1510.17994827976594807No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1510.17994827976594807No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1510.17994827976594807No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1450.1727980169937912No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1440.17160630653176503No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1430.1704145960697389No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1380.16445604375960818No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1280.15253893913934669No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAGACCCTGAT1220.14538867636718983No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1210.14419696590516368No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1200.14300525544313755No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1190.1418135449811114No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1190.1418135449811114No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT1150.13704670313300682No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1140.13585499267098067No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT1130.13466328220895452No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1090.12989644036084994No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1090.12989644036084994No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1090.12989644036084994No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT1060.12632130897477148No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1050.12512959851274535No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1040.1239378880507192No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1020.1215544671266669No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC990.11797933574058846No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT970.11559591481653617No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGAAACCCTGAT950.11321249389248389No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC930.11082907296843159No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGCACAATGGGGGAAACCCTGAT930.11082907296843159No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT930.11082907296843159No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC920.10963736250640545No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT900.10725394158235316No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAGACCCTGAT890.10606223112032699No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT880.10487052065830085No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT860.10248709973424858No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC840.10010367881019627No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT840.10010367881019627No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT840.10010367881019627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)