FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54754
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA9921.8117397815684697No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9781.7861708733608503No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9131.6674580852540453No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA8511.554224348906016No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA7861.435511560799211No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6991.2766190597947182No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6791.2400920480695474No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6641.2126967892756695No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA6351.1597326222741717No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5971.0903312999963473No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA5791.0574569894436936No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5661.0337144318223326No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4910.8967381378529423No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4620.8437739708514447No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4530.8273368155751177No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4520.8255104649888592No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4520.8255104649888592No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4400.8035942579537568No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2460.44928224421960045No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2160.39449172663184423No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1940.35431201373415644No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1890.34518026080286374No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1870.34152755963034664No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1600.2922160938013661No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1560.28491069145633197No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1470.2684735361800051No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1380.25203638090367825No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1360.24838367973116118No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1300.23742557621360993No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1200.21916207035102458No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA1190.21733571976476604No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1180.21550936917850752No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1170.21368301859224897No Hit
GACTACTGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1080.19724586331592212No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1080.19724586331592212No Hit
GACTACTCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA1060.19359316214340505No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1010.18446140921211235No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1010.18446140921211235No Hit
GACTACTAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA990.18080870803959528No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG920.16802425393578552No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG890.1625452021770099No Hit
GACTACCAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA860.1570661504182343No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA860.1570661504182343No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA850.15523979983197575No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA840.1534134492457172No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA830.15158709865945869No Hit
GACTACCCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA820.14976074807320014No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG790.1442816963144245No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA770.14062899514190744No Hit
GACTACTCGGGTATCTAATCCGGTTATAACACATCTGACGCTGCCGACGA770.14062899514190744No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA770.14062899514190744No Hit
GACTACTAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA760.1388026445556489No Hit
GACTACACGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA760.1388026445556489No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA760.1388026445556489No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA750.13697629396939037No Hit
GACTACCGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA740.13514994338313183No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC740.13514994338313183No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG730.13332359279687328No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG720.13149724221061476No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG720.13149724221061476No Hit
GACTACCCGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA710.12967089162435622No Hit
GACTACCAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA710.12967089162435622No Hit
GACTACTGGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA710.12967089162435622No Hit
GACTACTGGGGTATCTAATCCCGCGGTTACACATCTGACGCTGCCGACGA700.12784454103809767No Hit
GACTACAGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA700.12784454103809767No Hit
GACTACCGGGGTATCTAATCCGGTTATAACACATCTGACGCTGCCGACGA700.12784454103809767No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC690.12601819045183912No Hit
GACTACCGGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA680.12419183986558059No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA670.12236548927932206No Hit
GACTACTAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA670.12236548927932206No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA660.12053913869306351No Hit
GACTACTGGGGTATCTAATCCGGTTATAACACATCTGACGCTGCCGACGA660.12053913869306351No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA650.11871278810680497No Hit
GACTACTAGGGTATCTAATCCGGTTATAACACATCTGACGCTGCCGACGA650.11871278810680497No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG640.11688643752054645No Hit
GACTACAAGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA630.1150600869342879No Hit
GACTACTCGGGTATCTAATCCCGTGAAGACACATCTGACGCTGCCGACGA610.11140738576177084No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG610.11140738576177084No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC610.11140738576177084No Hit
GACTACCCGGGTATCTAATCCGGTTATAACACATCTGACGCTGCCGACGA600.10958103517551229No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC590.10775468458925376No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG590.10775468458925376No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG570.10410198341673667No Hit
GACTACAAGGGTATCTAATCCCGCAGTTACACATCTGACACTGCCGACGA570.10410198341673667No Hit
GACTACTCGGGTATCTAATCCCGCGGTTACACATCTGACGCTGCCGACGA570.10410198341673667No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG560.10227563283047815No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA560.10227563283047815No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA560.10227563283047815No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCCGACGCTGCCGACGA550.1004492822442196No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)