FastQCFastQC Report
Wed 8 May 2024
EGAF00008119539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100741
Total Bases30.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT27172.6970151179757993No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT19651.950546450799575No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16601.647789877011346No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16601.647789877011346No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15721.56043716063966No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11871.1782690265135347No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8700.863600718674621No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC8390.8328287390436864No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7010.6958437974608154No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC6110.6065057920806822No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC5970.5926087690215502No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC5940.5896308355088792No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC5540.5499250553399312No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC5190.5151824976921016No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4800.4764693620273771No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4800.4764693620273771No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC3820.3791902006134543No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3540.3513961544951906No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3480.34544028746984845No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT3320.32955797540226917No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3030.3007712847797818No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT2980.2958080622586633No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2890.28687426172065No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2860.2838963282079789No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC2760.27396988316574183No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2760.27396988316574183No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2700.2680140161403996No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2690.2670213716361759No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2630.2610655046108337No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT2510.24915377056014928No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2460.24419054803903076No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2250.22334501345033306No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2220.2203670799376619No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT2130.21143327939964862No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT2080.2064700568785301No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT2040.20249947886163527No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1910.18959510030672716No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1890.18760981129827975No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1880.18661716679405604No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1860.18463187778560863No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1830.18165394427293755No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1780.17669072175181902No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1740.17272014373492423No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1700.1687495657180294No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1690.1677569212138057No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1620.1608084096842398No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1600.15882312067579238No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1600.15882312067579238No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACG1590.15783047617156867No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1560.15485254265889759No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1520.15088196464200276No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1480.14691138662510794No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1460.14492609761666053No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1460.14492609761666053No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGTGGAT1440.14294080860821315No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1430.14194816410398944No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1400.13897023059131833No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1390.13797758608709462No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1380.13698494158287092No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT1360.13499965257442353No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1350.1340070080701998No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1340.13301436356597612No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1340.13301436356597612No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT1320.1310290745575287No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1310.130036430053305No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCACAATGGGGGAAACCCTGAT1310.130036430053305No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1300.1290437855490813No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1290.1280511410448576No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1290.1280511410448576No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1280.1270584965406339No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1280.1270584965406339No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1260.12507320753218648No Hit
CCTACGGGGGGCTGCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACG1250.12408056302796279No Hit
CCTACGGGTGGCTGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT1230.12209527401951538No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1220.12110262951529169No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1190.11812469600262059No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1190.11812469600262059No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1180.1171320514983969No Hit
CCTACGGGCGGCTGCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACG1170.11613940699417317No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1170.11613940699417317No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1170.11613940699417317No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1160.11514676248994948No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1150.11415411798572578No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1150.11415411798572578No Hit
CCTACGGGTGGCTGCAGTGGCGAAGGCGGATTGCTGGACGACAACTGACG1140.11316147348150206No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1140.11316147348150206No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1130.11216882897727837No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG1120.11117618447305468No Hit
CCTACGGGTGGCTGCAGTGGTGAATATTGCACAATGGGGGAAACCCTGAT1110.11018353996883096No Hit
CCTACGGGGGGCTGCAGTGGGGAACATTGCACAATGGGGGAAACCCTGAT1110.11018353996883096No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT1100.10919089546460727No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT1090.10819825096038355No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1080.10720560645615985No Hit
CCTACGGGTGGCTGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG1080.10720560645615985No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1070.10621296195193616No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAGACCCTGAT1050.10422767294348875No Hit
CCTACGGGGGGCAGCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACG1050.10422767294348875No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1040.10323502843926505No Hit
CCTACGGGTGGCTGCAGTGGGGAATATCGCACAATGGGGGAAACCCTGAT1030.10224238393504133No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCATTGTTGGAT1010.10025709492659395No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)