FastQCFastQC Report
Wed 8 May 2024
EGAF00008119543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131012
Total Bases39.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC72415.526974628278325No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC60074.585076176228132No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC50643.8652947821573593No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC43873.3485482245901137No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC42303.2287118737214913No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT29912.282996977376118No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC29102.2211705797942174No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC26892.0524837419473028No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT25561.9509663236955392No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC25371.936463835373859No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT24381.8608982383293133No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT22101.6868683784691478No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT18331.3991084786126462No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17841.3617073245198914No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16461.256373461972949No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16201.2365279516380179No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT15261.1647787988886513No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12100.9235795194333344No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT11290.8617531218514335No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10970.8373278783622875No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10260.7831343693707447No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10060.7678685921900283No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9970.7609989924587061No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7790.5946020211888987No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT5830.4449974048178793No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4810.3671419411962263No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT4800.36637865233719047No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT4640.3541660305926175No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT3950.30149909931914637No Hit
CCTACGGGCGGCTGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT3510.26791438952157054No Hit
CCTACGGGGGGCAGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT3310.2526486123408543No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3210.24501572375049616No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3200.2442524348914603No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3080.23509296858303055No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3020.23051323542881566No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2850.21753732482520685No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2480.18929563704088173No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT2260.17250328214209384No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2240.1709767044240222No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2040.15571092724330596No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2000.1526577718071627No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1970.15036790523005525No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT1900.14502488321680457No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1870.14273501663969712No Hit
CCTACGGGAGGCTGCAGTGGGGGATATTGCACAATGGGCGGAAGCCTGAT1870.14273501663969712No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1670.12746923945898087No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1630.1244160840228376No Hit
CCTACGGGTGGCTGNAGTAGGGAATCTTCCGCAATGGGCGAAAGCNTGAC1560.11907306200958691No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1500.11449332885537203No Hit
CCTACGGGGGGCTGNAGTAGGGAATCTTCCGCAATGGGCGAAAGCNTGAC1430.10915030684212133No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1400.10686044026501389No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1390.10609715140597809No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1370.10457057368790645No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1370.10457057368790645No Hit
CCTACGGGTGGCAGNAGTAGGGAATCTTCCGCAATGGGCGAAAGCNTGAC1350.10304399596983484No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)