FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131012
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG34872.6615882514578817No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG33532.5593075443470825No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG25711.9624156565810766No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG25191.9227246359112142No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG21461.6380178914908556No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG20181.5403169175342717No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG20161.5387903398162002No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC17331.3227795927090649No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG16361.248740573382591No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC15241.1632522211705798No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14831.1319573779501113No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG14811.1304308002320398No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG14341.0945562238573565No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12070.9212896528562269No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11220.8564100998381828No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10700.8167190791683204No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10520.8029798797056759No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9930.7579458370225628No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9870.753366103868348No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9030.6892498397093396No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8320.6350563307177968No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8280.6320031752816536No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7920.6045247763563643No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7780.593838732329863No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7470.5701767776997527No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7350.5610173113913229No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5970.4556834488443806No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG3820.29157634415168077No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG3540.270204256098678No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG2660.2030348365035264No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG2600.19845510334931152No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG2530.1931120813360608No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG2470.18853234818184594No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG2400.18318932616859523No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG2140.16334381583366409No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG2010.1534210606661985No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG1850.1412084389216255No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG1790.13662870576741062No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTNTTCCTCCACGCGGCGTCGC1770.135102128049339No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1760.13433883919030318No Hit
GACTACACGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG1690.12899581717705247No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG1590.12136292858669435No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG1540.11754648429151528No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1520.11601990657344366No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGCTCCTCAG1500.11449332885537203No Hit
GACTACTCGGGTATCTAATCCTGTTTGNTCCCCACGCTTTCGCACATCAG1500.11449332885537203No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG1490.11373003999633621No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG1470.11220346227826458No Hit
GACTACTGGGGTATCTAATCCTGTTTGNTCNCCACGCTTTCGCACATCAG1460.11144017341922878No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTNTTCCTCCACGCGGCGTCGC1450.11067688456019295No Hit
GACTACTCGGGTATCTAATCCTGTTTGNTCNCCACGCTTTCGCACATCAG1360.10380728482887064No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG1320.10075412939272738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)