FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008119555

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119555
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52744
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT26785.07735477021083No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT20163.822235704535113No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT19983.788108600030335No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17943.401334748976187No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT15642.9652661914151373No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12052.2846200515698465No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT9661.8314879417564083No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT5941.1261944486576672No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT4390.8323221598665251No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4370.8285302593659942No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT3570.6768542393447596No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2960.5612012740785681No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2920.5536174730775064No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2810.5327620203245867No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2740.5194903685727287No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2660.5043227665706052No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2660.5043227665706052No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2580.48915516456848174No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2470.46829971181556196No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2400.45502806006370394No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2110.40004550280600637No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2030.3848779008038829No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1900.36023054755043227No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1790.3393750947975125No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1630.3090398907932656No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1630.3090398907932656No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1570.297664189291673No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1540.2919763385408767No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1520.2881844380403458No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1490.2824965872895495No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1480.28060063703928406No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1330.2521613832853026No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1270.24078568178371001No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1230.23320188078264828No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1210.2294099802821174No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1210.2294099802821174No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT1150.2180342787805248No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1090.2066585772789322No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1070.2028666767784013No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1060.20097072652813588No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1040.19717882602760503No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1030.1952828757773396No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT990.18769907477627787No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT970.183907174275747No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT940.1782193235249507No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC940.1782193235249507No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT920.17442742302441983No Hit
CCTACGGGTGGCTGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT920.17442742302441983No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC910.1725314727741544No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT910.1725314727741544No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT890.16873957227362354No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT890.16873957227362354No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT880.16684362202335812No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT840.15925982102229638No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC830.15736387077203096No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT800.15167602002123465No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTTCGAATGGGAT780.1478841195207038No Hit
CCTACGGGTAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT770.14598816927043834No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT770.14598816927043834No Hit
CCTACGGGTGGCAGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT760.1440922190201729No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT750.14219626876990746No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT730.1384043682693766No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTATGATATCAGGAT720.13650841801911118No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT720.13650841801911118No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT710.13461246776884575No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT710.13461246776884575No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC710.13461246776884575No Hit
CCTACGGGGAGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT700.1327165175185803No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC700.1327165175185803No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT690.13082056726831487No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTTGGACTGGAT690.13082056726831487No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG680.12892461701804944No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT680.12892461701804944No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT670.12702866676778402No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT670.12702866676778402No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC670.12702866676778402No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT660.1251327165175186No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT660.1251327165175186No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC660.1251327165175186No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT650.12323676626725315No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT650.12323676626725315No Hit
CCTACGGGTAGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT650.12323676626725315No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT650.12323676626725315No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTGTTAATTGGAT630.11944486576672228No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT630.11944486576672228No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT610.11565296526619143No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT610.11565296526619143No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT600.11375701501592599No Hit
CCTACGGGTGGCTGCAGTCGTCGGCGGCGTCAGATGTGTTATAACCGGAT600.11375701501592599No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT590.11186106476566056No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGACGTGTACATAGCGGAT590.11186106476566056No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT580.10996511451539512No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT580.10996511451539512No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT570.10806916426512969No Hit
CCTACGGGCGGCTGCAGACGTCGGCAGCGTCAGATGTATGATATCGGGAT570.10806916426512969No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT560.10617321401486426No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCTTACAGGAT560.10617321401486426No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC560.10617321401486426No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGACGTGTCTTCACGGGAT550.10427726376459881No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATATGTCTTCACGGGAT550.10427726376459881No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCGGATGTGTAGACTTGGGAT550.10427726376459881No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTATAAGAGACAG550.10427726376459881No Hit
CATACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT540.10238131351433337No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT540.10238131351433337No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT530.10048536326406794No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTTCACGCCGGAT530.10048536326406794No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACTGCGGGAT530.10048536326406794No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT530.10048536326406794No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGCTCTCTGGTCTGTAACTGACG530.10048536326406794No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)