FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119582

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119582
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95227
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG31743.3330883047875077No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30563.2091738687557103No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG24782.602203156667752No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG23612.479338842975207No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20582.161151774181692No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18751.9689793860984806No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG18121.9028216787255716No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG15621.6402910939124407No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG13311.3977128335451081No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8030.8432482384197758No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7030.7382360044945236No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6120.642674871622544No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5940.6237726695159986No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5020.5271614143047665No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4980.5229609249477564No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4570.47990590903840297No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG4380.45995358459260505No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4330.45470297289634243No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4240.4452518718430697No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4160.4368508931290495No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG4020.4221491803795142No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG3820.4011467335944638No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG3750.3937958772196961No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3730.391695632541191No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG3670.38539489850567593No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3640.3822445314879183No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3510.3685929410776355No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG3500.367542818738383No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG3500.367542818738383No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG3450.3622922070421204No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG3410.35809171768511033No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3340.35074086131034266No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG3320.3486406166318376No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG3290.34549024961408004No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3280.34444012727482753No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG3250.3412897602570699No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG3200.3360391485608073No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG3190.3349890262215548No Hit
GANTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3130.3286882921860397No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG3110.3265880475075346No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG3060.321337435811272No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG3050.3202873134720195No Hit
NNCTNNCGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG3030.31818706879351444No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG3010.31608682411500943No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG3000.3150367017757569No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2980.3129364570972518No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2940.3087359677402417No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2930.3076858454009892No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2910.3055856007224842No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2900.30453547838323164No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2890.3034853560439791No Hit
NNNTNNCCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2880.3024352337047266No Hit
NANTANCGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2830.297184622008464No Hit
NNCTNNTAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2830.297184622008464No Hit
GNNTANCGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2820.29613449966921146No Hit
NANTANTAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2720.2856332762766862No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2690.28248290925892866No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2690.28248290925892866No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2640.27723229756266604No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2640.27723229756266604No Hit
GANTNNCCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2570.2698814411878984No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2560.26883131884864586No Hit
GANTNNTAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2520.2646308294916358No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2480.2604303401346257No Hit
NNNTNNCAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2470.2593802177953732No Hit
NANTNNCAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2430.25517972843836306No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2420.25412960609911056No Hit
GANTNNCAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG2400.2520293614206055No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2380.24992911674210044No Hit
NANTNNAGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG2370.24887899440284794No Hit
NNNTNNCCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2340.24572862738509038No Hit
GNNTANCCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2340.24572862738509038No Hit
GANTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2330.24467850504583782No Hit
NNNTNNAGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG2300.24152813802808026No Hit
NNNTNNCAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG2250.23627752633181764No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2230.2341772816533126No Hit
NNCTNNCAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2150.2257763029392924No Hit
NNCTNNCCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG2120.22262593592153485No Hit
GNNTANCAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG2100.22052569124302981No Hit
GANTANCCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG2080.21842544656452476No Hit
GANTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2070.21737532422527223No Hit
NANTANCCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG2050.2152750795467672No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2040.2142249572075147No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2010.2110745901897571No Hit
NANTNNACGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG1980.20792422317199954No Hit
NANTANCAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1950.20477385615424196No Hit
NNCTNNACGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG1940.20372373381498943No Hit
NANTANAGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1930.20267361147573693No Hit
GANTANAGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1930.20267361147573693No Hit
GANTANCAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1910.2005733667972319No Hit
NNCTNNAGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG1890.1984731221187268No Hit
GANTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1890.1984731221187268No Hit
GNNTANACGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1820.19112226574395916No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1810.19007214340470666No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1800.18902202106545413No Hit
GANTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1700.17852079767292892No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1680.17642055299442386No Hit
NNNTNNACGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG1680.17642055299442386No Hit
NANTNNAAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG1610.1690696966196562No Hit
NNNTNNACGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1570.16486920726264612No Hit
GNNTANAAGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1550.16276896258414106No Hit
GNNTANAGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG1540.16171884024488853No Hit
GANTANACGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1540.16171884024488853No Hit
NNNTNNAGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1530.160668717905636No Hit
NANTANACGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1530.160668717905636No Hit
NNNTNNAAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG1430.1501674945131108No Hit
GANTNNAGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1430.1501674945131108No Hit
NNCTNNAAGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG1420.14911737217385826No Hit
NNNTNNAAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG1420.14911737217385826No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1340.14071639345983808No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1320.13861614878133302No Hit
GANTNNACGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1260.1323154147458179No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1250.13126529240656537No Hit
GANTANAAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG1230.1291650477280603No Hit
GANTNNAAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG1200.12601468071030275No Hit
NANTANAAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG1170.12286431369254519No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1000.10501223392525229No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)