FastQCFastQC Report
Wed 8 May 2024
EGAF00008119583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34151
Total Bases10.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11283.302977950865275No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC8082.365962929343211No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7182.1024274545401305No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC7052.0643612192907965No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7032.058504875406284No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6882.014582296272437No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6721.967731545196334No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5881.7217651020467923No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5231.531433925800123No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5091.4904395186085326No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4841.4172352200521214No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4831.414307048109865No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4781.3996661883985828No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT4571.3381745776111973No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4331.2678984509970426No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4031.180053292729349No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC3951.1566279171912974No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3951.1566279171912974No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3881.1361307135955023No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3501.0248601797897572No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3501.0248601797897572No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3501.0248601797897572No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT3431.004362976193962No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3080.9018769582149864No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3050.8930924423882171No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2590.7583965330444203No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2350.6881204064302656No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2320.6793358906034963No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC2290.6705513747767269No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2270.664695030892214No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2140.6266287956428801No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2060.6032034201048285No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC2040.5973470762203157No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1940.5680653567977512No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1880.5504963251442124No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1770.5182864337793915No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1750.5124300898948786No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1570.45972299493426255No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1540.4509384791074932No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1530.4480103071652367No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1530.4480103071652367No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1430.41872858774267224No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1380.40408772803139No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1360.3982313841468771No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1320.3865186963778513No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1280.3748060086088255No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC1270.3718778366665691No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1240.3630933208397997No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1210.35430880501303036No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1120.3279552575327223No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1100.3220989136482094No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC1040.30452988199467074No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1020.29867353811015784No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT970.2840326783988756No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT940.2752481625721062No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC900.2635354748030804No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC870.2547509589763111No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT810.2371819273227724No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC810.2371819273227724No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT750.21961289566923367No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT740.21668472372697722No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC730.2137565517847208No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT720.21082837984246436No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT720.21082837984246436No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC720.21082837984246436No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT700.20497203595795147No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC690.202043864015695No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC670.1961875201311821No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC650.1903311762466692No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT640.18740300430441276No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC640.18740300430441276No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT640.18740300430441276No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC630.1844748323621563No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT630.1844748323621563No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT630.1844748323621563No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT620.18154666041989986No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT620.18154666041989986No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA610.17861848847764342No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA610.17861848847764342No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT600.17569031653538697No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT600.17569031653538697No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC600.17569031653538697No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT590.1727621445931305No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT560.16397762876636116No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT560.16397762876636116No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT560.16397762876636116No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA550.1610494568241047No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT550.1610494568241047No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT550.1610494568241047No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC540.15812128488184826No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC540.15812128488184826No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT530.15519311293959182No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC520.15226494099733537No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT520.15226494099733537No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA520.15226494099733537No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA510.14933676905507892No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT510.14933676905507892No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC490.14348042517056603No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC490.14348042517056603No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC480.14055225322830955No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT470.1376240812860531No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT470.1376240812860531No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA470.1376240812860531No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT460.1346959093437967No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT460.1346959093437967No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT440.12883956545928377No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC440.12883956545928377No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT440.12883956545928377No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC440.12883956545928377No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC430.12591139351702732No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC420.12298322157477087No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA420.12298322157477087No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT420.12298322157477087No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC410.12005504963251443No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT400.11712687769025797No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC400.11712687769025797No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC400.11712687769025797No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT390.11419870574800152No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTGCACAATGGGCGAAAGCCTGAT390.11419870574800152No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT380.11127053380574509No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT370.10834236186348861No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT370.10834236186348861No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT370.10834236186348861No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGGGCAACCCTGAT370.10834236186348861No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCAAGAGTCTGA360.10541418992123218No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT360.10541418992123218No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC360.10541418992123218No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGCGCAAGCCTGAT350.10248601797897573No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGAAAGTCTGAC350.10248601797897573No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCGCAATGGGGGGAACCCTGAC350.10248601797897573No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT350.10248601797897573No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT350.10248601797897573No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)