FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121644
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC15751.2947617638354543No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT14571.1977573904179408No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT10640.8746835026799513No Hit
CCTACGGGGGGCAGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC10020.8237151030876985No Hit
CCTACGGGTGGCTGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC9960.8187826773207063No Hit
CCTACGGGGGGCTGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC9620.7908322646410839No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC9520.7826115550294301No Hit
CCTACGGGTGGCAGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC9090.7472625036993193No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC9030.7423300779323272No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8450.6946499621847357No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8330.6847851106507514No Hit
CCTACGGGCGGCAGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC8060.6625891946992865No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7930.6519022722041367No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7780.6395712077866562No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7580.6231297885633488No Hit
CCTACGGGCGGCTGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC6950.5713393180099305No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT6480.5327019828351583No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6420.5277695570681662No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC6310.5187267764953472No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6080.49981914438854363No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6010.49406464766038605No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5980.49159843477688997No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC5960.48995429285455916No Hit
CCTACGGGAGGCTGCAGTGATAAATATTAAGCAATGAACGAAAGTTTGAC5950.4891322218933939No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC5850.4809115122817401No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5730.4710466607477558No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5730.4710466607477558No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5420.4455624609516293No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC5370.4414521061458025No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5370.4414521061458025No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5360.44063003518463717No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5250.4315872546118181No Hit
CCTACGGGTGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5090.4184341192331722No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5060.4159679063496761No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4660.3830850679030614No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4340.3567787971457696No Hit
CCTACGGGCGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4280.3518463713787774No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC4110.3378711650389662No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4030.3312945973496432No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3990.3280063135049817No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3980.3271842425438164No Hit
CCTACGGGAGGCTGCAGTGGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3950.32471802966032026No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3750.30827661043701293No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3660.3008779717865246No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT3400.27950412679622505No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTCCGCAATGGGCGAAAGCCTGAC3390.27868205583505967No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3370.2770379139127289No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3170.2605964946894216No Hit
CCTACGGGAGGCAGCAGTGGGGAAGGCGGCTTTCTGGACTGTAACTGACG3080.2531978560389333No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2970.24415507546611423No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2770.2277136562428069No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT2760.2268915852816415No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT2720.22360330143698007No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT2660.21867087566998783No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT2640.2170267337476571No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT2620.21538259182532637No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT2550.2096280950971688No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2490.20469566933017658No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2380.19565288875735753No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT2350.19318667587386143No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2250.18496596626220774No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2210.18167768241754628No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2170.1783893985728848No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2090.17181283088356186No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2090.17181283088356186No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT2060.16934661800006576No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT2050.1685245470389004No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2000.16441419223307358No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT2000.16441419223307358No Hit
CCTACGGGAGGCAGCAGAGGAGAAAGGGGTGGAGGGAGGCTGGCTCTTCT1990.1635921212719082No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT1960.1611259083884121No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCGGTAGTCCTGTCTCT1920.1578376245437506No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT1900.15619348262141988No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1890.15537141166025453No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1880.15454934069908915No Hit
CCTACGGGTGGCTGCAGTGGGGAAGGCGGCTTTCTGGACTGTAACTGACG1810.14879484397093157No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1790.14715070204860084No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1790.14715070204860084No Hit
CCTACGGGGGGCTGCAGTGGGGAAGGCGGCTTTCTGGACTGTAACTGACG1790.14715070204860084No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGAGTAGTCCTGTCTCT1780.14632863108743546No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1760.14468448916510473No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1730.14221827628160863No Hit
CCTACGGGTGGCAGCAGTGGGGAAGGCGGCTTTCTGGACTGTAACTGACG1710.14057413435927787No Hit
CCTACGGGCGGCTGCAGTGGGGAAGGCGGCTTTCTGGACTGTAACTGACG1690.13892999243694718No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1650.1356417085922857No Hit
CCTACGGGCGGCAGCAGTGGGGAAGGCGGCTTTCTGGACTGTAACTGACG1640.13481963763112031No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1630.13399756666995494No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1620.1331754957087896No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1620.1331754957087896No Hit
CCTACGGGAGGCAGCAGTGGGGAATACTGCACAATGGGGGAAACCCTGAT1590.13070928282529348No Hit
CCTACGGGGGGCAGCAGTGGGGAAGGCGGCTTTCTGGACTGTAACTGACG1590.13070928282529348No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTAGTAGTCCTGTCTCT1550.127420998980632No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTGGTAGTCCTGTCTCT1540.12659892801946665No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1510.12413271513597053No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1500.12331064417480517No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCGTGGG1480.12166650225247443No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1470.12084443129130908No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1470.12084443129130908No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCTGTAGTCCTGTCTCT1470.12084443129130908No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1460.12002236033014371No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1450.11920028936897833No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTCTTCACGGGAT1450.11920028936897833No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1430.1175561474466476No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1430.1175561474466476No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1420.11673407648548223No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1420.11673407648548223No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1380.11344579264082075No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1370.11262372167965538No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGTGTAGTCCTGTCTCT1370.11262372167965538No Hit
CCTACGGGAGGCTGCAGTGGGGAAGGCGGCTTTCTGGACTGTAACTGACG1370.11262372167965538No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1370.11262372167965538No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT1340.11015750879615929No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCCGGTAGTCCTGTCTCTTATAC1340.11015750879615929No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1330.10933543783499391No Hit
CCTACGGGTGGCAGCAGAGGAGAAAGGGGTGGAGGGAGGCTGGCTCTTCT1320.10851336687382855No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1310.10769129591266319No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1300.10686922495149781No Hit
CCTACGGGCGGCAGCAGAGGAGAAAGGGGTGGAGGGAGGCTGGCTCTTCT1300.10686922495149781No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1290.10604715399033245No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1290.10604715399033245No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1280.10522508302916707No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1280.10522508302916707No Hit
CCTACGGGCGGCAGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT1270.1044030120680017No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1270.1044030120680017No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1270.1044030120680017No Hit
CCTACGGGGGGCTGCAGAGGAGAAAGGGGTGGAGGGAGGCTGGCTCTTCT1270.1044030120680017No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1260.10358094110683633No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1250.10275887014567098No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1250.10275887014567098No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1240.10193679918450561No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1240.10193679918450561No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1230.10111472822334025No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1230.10111472822334025No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT1230.10111472822334025No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1220.10029265726217487No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCTTGTAGTCCTGTCTCTTATAC1220.10029265726217487No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)