FastQCFastQC Report
Wed 8 May 2024
EGAF00008119608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121644
Total Bases36.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT10270.8442668771168328No Hit
GACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT9710.7982309032915721No Hit
GACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT8410.6913616783400742No Hit
GACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT8060.6625891946992865No Hit
GACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT7950.6535464141264674No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG7660.6297063562526717No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7420.6099766531847028No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG7390.6075104403012068No Hit
GACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT7290.5992897306895532No Hit
GACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT6960.572161388971096No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG6770.556542040708954No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6640.5458551182138043No Hit
GACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT6590.5417447634079774No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG6520.5359902666798197No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG6440.5294136989904968No Hit
GACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATCGTGCATTAT6260.5146164216895203No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6190.5088619249613626No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6160.5063957120778666No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6050.4973529315050475No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG5930.4874880799710631No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG5880.4833777251652363No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG5690.4677583769030943No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5660.4652921640195982No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5650.46447009305843284No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5650.46447009305843284No Hit
GACTACACGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG5640.4636480220972674No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5470.4496728157574562No Hit
GACTACTGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG5270.4332313965341488No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTAGCTTTCGAGCCTCAG5130.4217224030778337No Hit
GACTACCGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG5080.4176120482720068No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5020.4126796225050146No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5010.41185755154384923No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG5010.41185755154384923No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4950.4069251257768571No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4700.38637335174772286No Hit
GACTACTCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG4670.3839071388642268No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4610.3789747130972345No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4590.3773305711749038No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG4490.36910986156325015No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4470.3674657196409194No Hit
GACTACAGGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG4380.3600670809904311No Hit
GACTACAAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG4350.35760086810693503No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4190.3444477327282891No Hit
GACTACCAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3780.31074282332050907No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3670.30170004274769No Hit
GACTACCCGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3660.3008779717865246No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3610.29676761698069776No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3590.29512347505836706No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3380.27785998487389435No Hit
GACTACTAGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG3270.2688172043010753No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3030.24908750123310647No Hit
GACTACACGGGTATCTAATCCCATTTGCTCCCCTAGCTTTCGTCTCTCAG2970.24415507546611423No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2960.24333300450494885No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2930.24086679162145275No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2840.23346815297096446No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT2740.22524744335931077No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2720.22360330143698007No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT2610.21456052086416103No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT2610.21456052086416103No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2600.21373844990299562No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT2550.2096280950971688No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT2510.2063398112525073No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT2500.20551774029134195No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2470.20305152740784585No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2420.19894117260201902No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2360.19400874683502678No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2290.18825425010686922No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT2270.1866101081845385No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2190.18003354049521555No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2150.17674525665055407No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA2140.1759231856893887No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2130.17510111472822332No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2060.16934661800006576No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2030.16688040511656965No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2030.16688040511656965No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT2010.16523626319423892No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT1990.1635921212719082No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1970.16194797934957747No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1910.15701555358258523No Hit
GACTACTGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT1880.15454934069908915No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT1840.15126105685442767No Hit
GACTACCGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT1770.1455065601262701No Hit
GACTACCCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT1770.1455065601262701No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1750.14386241820393936No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1740.143040347242774No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1730.14221827628160863No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1720.14139620532044325No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1710.14057413435927787No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1670.13728585051461642No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT1660.13646377955345107No Hit
GACTACTCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT1660.13646377955345107No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1630.13399756666995494No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1630.13399756666995494No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1620.1331754957087896No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1600.13153135378645883No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1600.13153135378645883No Hit
GACTACTAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT1580.12988721186412813No Hit
GACTACAGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT1560.12824306994179738No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1530.12577685705830127No Hit
GACTACCAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT1520.12495478609713591No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT1510.12413271513597053No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1510.12413271513597053No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1500.12331064417480517No Hit
GACTACACGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT1440.11837821840781297No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1440.11837821840781297No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT1430.1175561474466476No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1430.1175561474466476No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1420.11673407648548223No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT1410.11591200552431685No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1400.11508993456315149No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1400.11508993456315149No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1400.11508993456315149No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT1390.11426786360198612No Hit
GACTACCGGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC1380.11344579264082075No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT1340.11015750879615929No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1330.10933543783499391No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT1330.10933543783499391No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1320.10851336687382855No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT1300.10686922495149781No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTGCGCCCATTGT1290.10604715399033245No Hit
GACTACAAGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC1280.10522508302916707No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT1280.10522508302916707No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1280.10522508302916707No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1280.10522508302916707No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT1270.1044030120680017No Hit
GACTACTGGGGTATCTAATCCTGCTGCCTCCCGTAGGCTGTCTCTTATAC1260.10358094110683633No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1250.10275887014567098No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT1250.10275887014567098No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1240.10193679918450561No Hit
GACTACAAGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT1240.10193679918450561No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT1230.10111472822334025No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT1230.10111472822334025No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCGCCCGTAGGCTGTCTCT1230.10111472822334025No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)