FastQCFastQC Report
Wed 8 May 2024
EGAF00008119627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45797
Total Bases13.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT40318.801886586457629No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT24845.423936065681159No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23975.233967290433871No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT23315.089853047142826No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT22935.0068781797934365No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT22684.952289451274101No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT18824.109439482935564No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT18193.9718758870668385No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15013.277507260300893No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT14153.0897220341943794No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13272.8975697098063193No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13182.8779177675393584No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12662.764373212219141No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10542.3014607943751773No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10112.207568181321921No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8631.8844029084874554No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4160.9083564425617398No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2340.5109504989409787No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2190.47819726182937744No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2130.465095966984737No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2050.4476275738585497No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1980.43234272987313577No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1770.38648819791689415No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1680.3668362556499334No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1510.32971592025678537No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1450.3166146254121449No Hit
CCTACGGGGTGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1160.25329170032971593No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1140.2489246020481691No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1130.24674105290739567No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1110.24237395462584885No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1090.23800685634430205No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1060.23145620892198177No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1060.23145620892198177No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1000.2183549140773413No Hit
ACTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT990.21617136493656788No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT930.20307007009192743No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC930.20307007009192743No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC900.19651942266960715No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC880.19215232438806035No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT850.18560167696574012No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC820.17905102954341987No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC790.17250038212109964No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT790.17250038212109964No Hit
CCTACGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATG780.17031683298032624No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC740.16158263641723256No Hit
ACTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT690.1506648907133655No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT680.1484813415725921No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC680.1484813415725921No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC680.1484813415725921No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT660.14411424329104525No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT660.14411424329104525No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT650.14193069415027187No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC620.13538004672795162No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT610.1331964975871782No Hit
CCTACGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATG610.1331964975871782No Hit
CCTACGGGGAGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT600.1310129484464048No Hit
CCTACGGGGTGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT580.12664585016485796No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC570.12446230102408455No Hit
ACTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT560.12227875188331114No Hit
ACTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT540.11791165360176431No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT540.11791165360176431No Hit
ACTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT520.11354455532021747No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT510.11136100617944408No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT500.10917745703867066No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC500.10917745703867066No Hit
ACTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT490.10699390789789724No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC480.10481035875712383No Hit
CCTACGGGGCGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT460.100443260475577No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT460.100443260475577No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)