FastQCFastQC Report
Wed 8 May 2024
EGAF00008119654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77026
Total Bases23.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG31854.134967413600602No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG30914.012930698725105No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG30613.9739828109988835No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG29183.7883312128372237No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG26403.427414119907564No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG25963.3702905512424377No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG24803.219692052034378No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG24743.211902474489133No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG24613.195025056474437No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG24393.166463272141874No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG22082.866564536649962No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG21922.8457923298626437No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG21832.8341079635447772No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG21482.7886687611975174No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20922.715966037441903No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG20782.697790356502999No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG20332.6393685249136656No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG19702.5575779606885987No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7871.021732921351232No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7700.9996624516397061No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7470.9698024043829356No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7280.9451354088229948No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6700.869836159218965No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6120.7945369096149352No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5700.740009866798224No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5530.717939397086698No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5230.6789915093604757No Hit
GACTACTGGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG2620.3401448861423415No Hit
GACTACCGGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG2560.33235530859709705No Hit
GACTACTCGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG2260.2934074208708748No Hit
GACTACAGGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1990.2583543219172747No Hit
GACTACCAGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1890.24537169267520056No Hit
GACTACAAGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1860.24147690390257837No Hit
GACTACCCGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1690.21940643419105238No Hit
GACTACACGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1610.20902033079739307No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1590.20642380494897827No Hit
GACTACTAGGGTATCTAATCCTGTTTGACCCCCACGCTTTCGCACATCAG1520.19733596447952642No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1520.19733596447952642No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1510.196037701555319No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1470.19084464985848934No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1400.18175680938903746No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1370.17786202061641523No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1330.1726689689195856No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG1290.16747591722275595No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1280.16617765429854853No Hit
GACTACCTGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1250.1622828655259263No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1240.1609846026017189No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1240.1609846026017189No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG1200.15579155090488928No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1190.15449328798068185No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1190.15449328798068185No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1180.15319502505647442No Hit
GACTACTTGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1160.15059849920805962No Hit
GACTACATGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1130.1467037104354374No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1130.1467037104354374No Hit
GACTACATGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1110.14410718458702257No Hit
GACTACTTGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1090.14151065873860774No Hit
GACTACCTGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1020.13242281826915586No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG980.12722976657232624No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG970.1259315036481188No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG960.12463324072391141No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG950.123334977799704No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG900.11684366317866693No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG850.11035234855762989No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG820.10645755978500766No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG820.10645755978500766No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG810.10515929686080025No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG790.10256277101238544No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)