FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51016
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT16683.269562490199153No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC10742.1052218911713974No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10091.977810882860279No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9621.8856829230045475No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT9531.8680413987768545No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8991.7621922534106949No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8841.7327897130312058No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT8371.6406617531754744No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7141.3995609220636662No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7091.3897600752705033No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6971.3662380429669123No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6511.2760702524698135No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6461.2662694056766506No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6301.234906695938529No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5181.0153677277716795No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC3870.7585855417908107No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT3710.7272228320526893No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT3590.7037007997490984No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3270.6409753802728556No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2220.435157597616434No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2080.4077152265955779No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2080.4077152265955779No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2080.4077152265955779No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2030.39791437980241495No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2020.3959542104437824No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1980.388113533009252No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1980.388113533009252No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1960.38419319429198684No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1940.3802728555747216No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1890.3704720087815587No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1790.3508703151952329No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1770.3469499764779677No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1730.33910929904343734No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1670.32734828289164186No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1660.3253881135330092No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1620.3175474360984789No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1620.3175474360984789No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1470.2881448957189901No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1430.2803042182844598No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1130.2214991375254822No Hit
CCTACGGGAGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC980.19209659714599342No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT960.18817625842872823No Hit
CCTACGGGAGGCAGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG950.18621608907009565No Hit
CCTACGGGAGGCAGCAGTGGGGAAGGCGGATTGCTGGACAGTAACTGACG750.14701270189744392No Hit
CCTACGGGTGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC730.14309236318017876No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC660.12937117766975065No Hit
CCTACGGGGGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC660.12937117766975065No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT640.1254508389524855No Hit
CCTACGGGAGGCAGCAGTGGGGGATATTGGTCAATGGGGGAAACCCTGAA620.12153050023522032No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT610.11957033087658775No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT610.11957033087658775No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT600.11761016151795516No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT600.11761016151795516No Hit
CCTACGGGAGGCAGCAGTGGGGAAGGCGGATTGCTGGACGACAACTGACG580.11368982280068997No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC580.11368982280068997No Hit
CCTACGGGAGGCAGCAGTGGCGAAGGCGGATTGCTGGACAGTAACTGACG580.11368982280068997No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT570.1117296534420574No Hit
CCTACGGGGGGCTGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC560.10976948408342481No Hit
CCTACGGGCGGCAGCAGTGGGGAATCTTCCGCAATGGACGAAAGTCTGAC550.10780931472479222No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA530.10388897600752706No Hit
CCTACGGGTGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT530.10388897600752706No Hit
CCTACGGGCGGCAGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT530.10388897600752706No Hit
CCTACGGGCGGCAGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG520.10192880664889448No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)