FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107817
Sequences flagged as poor quality0
Sequence length301
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT81627.570234749622045No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT50754.707049908641495No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT49624.602242689000807No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT48184.468683046272851No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT45544.223823701271599No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT42853.974326868675626No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT37613.488318168748899No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT29152.703655267722159No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20291.8818924659376537No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT12561.1649368837938359No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC12471.1565894061233386No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12271.138039455744456No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12101.1222719979224056No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12021.1148520177708525No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT11421.0592021666342042No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC10360.9608874296261257No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT10240.9497574593987961No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9660.8959626033000361No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC8200.7605479655341922No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7820.725303059814315No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7620.7067531094354322No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC7450.690985651613382No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7420.6882031590565495No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7260.6733631987534433No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT7080.6566682434124489No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6870.637190795514622No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6810.6316258104009572No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6680.6195683426546834No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6660.6177133476167951No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6640.6158583525789069No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT6450.5982358997189683No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC6340.5880334270105827No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC6300.5843234369348062No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5810.5388760585065435No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5800.5379485609875994No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC5790.5370210634686552No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5600.5193986106087166No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT5400.5008486602298339No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5030.46653125202890083No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC4960.46003876939629185No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4770.44241631653635327No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4640.4303588487900795No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4450.41273639593014083No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4330.4016064257028112No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4260.3951139430702023No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4180.38769396291864916No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4080.37841898772920785No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4060.3765639926913195No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT4060.3765639926913195No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4040.3747089976534313No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3760.34873906712299546No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3550.3292616192251686No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3520.3264791266683362No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3110.2884517283916266No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2860.26526429041802313No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2690.24949683259597283No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2640.24485934500125214No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT2380.22074440950870458No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT2070.19199198642143633No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1920.17807952363727428No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1860.17251453852360946No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1830.16973204596677705No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1770.16416706085311222No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1720.1595295732583915No Hit
CCTACGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATG1640.15210959310683844No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1610.14932710055000603No Hit
CCTACGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATG1530.14190712039845294No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCTTGAC1480.13726963280373225No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1460.13541463776584398No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1450.13448714024689984No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGGGCAACCCTGAT1420.13170464769006743No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1350.12521216505745847No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1270.11779218490590537No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1240.11500969234907296No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1180.10944470723540814No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1170.10851720971646402No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT1120.10387972212174332No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCTTGAC1100.10202472708385506No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1080.10016973204596678No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)