FastQCFastQC Report
Wed 8 May 2024
EGAF00008119707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97004
Total Bases29.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT71947.416189023133067No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT43984.533833656344068No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT42714.40291122015587No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC42294.359614036534576No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT41244.251371077481341No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT40104.133850150509257No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT37473.862727310214012No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT30793.1740959135705746No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC26302.7112284029524556No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC24782.554533833656344No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC24672.5431940950888623No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC24402.515360191332316No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT24232.4978351408189354No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC23502.422580512143829No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC19351.9947631025524721No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC14941.5401426745288855No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC14901.5360191332316193No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC9991.0298544389922064No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC8780.9051173147499073No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC8300.8556348191827141No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC8070.8319244567234341No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC8000.8247082594532185No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6760.6968784792379695No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT5770.5948208321306339No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC5160.5319368273473258No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT5150.5309059420230093No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT3630.37421137272689786No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT3530.3639025194837326No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT3510.36184074883509953No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT3440.3546245515648839No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT3240.33400684507855344No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT3060.31545090924085606No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2910.29998762937610823No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT2880.2968949734031586No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2830.291740546781576No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2730.2814316935384108No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2510.25875221640344725No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2370.24431982186301598No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2290.23607273926848377No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT2220.22885654199826813No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGAGGGAACTCTGAT2080.21442414745783678No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1950.20102263824172198No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1920.19792998226877243No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1820.18762112902560718No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1790.18452847305265763No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1720.17731227578244196No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1640.16906519318790977No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1570.1618489959176941No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1570.1618489959176941No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1560.16081811059337758No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1530.15772545462042803No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1500.15463279864747845No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1500.15463279864747845No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1490.15360191332316192No Hit
CCTACGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATG1470.1515401426745289No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1430.14741660137726278No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1420.14638571605294626No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1380.14226217475568018No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1290.13298420683683146No Hit
CCTACGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGATG1280.13195332151251496No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1280.13195332151251496No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCTTGAC1240.12782978021524885No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1230.12679889489093232No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1230.12679889489093232No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGCACAATGGGGGAAACCCTGAT1150.11855181229640013No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1110.11442827099913407No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1090.11236650035050101No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA1080.11133561502618448No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1080.11133561502618448No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1070.11030472970186796No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1010.10411941775596883No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC980.10102676178301927No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)