FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40447
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5661.399362128217173No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5481.3548594456943654No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4981.2312408831310109No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4971.2287685118797438No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4901.2114619131208741No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4521.117511805572725No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4521.117511805572725No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4461.1026775780651223No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4151.0260340692758425No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4000.988948500506836No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3790.9370287042302272No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3650.9024155067124879No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3640.8999431354612208No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3450.8529680816871461No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3140.7763245728978663No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3110.768907459144065No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2980.7367666328775929No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2960.7318218903750587No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2950.7293495191237916No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2770.684846836600984No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2710.6700126090933815No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2480.6131480703142383No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2480.6131480703142383No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2390.5908967290528345No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2220.5488664177812941No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2040.5043637352584864No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2020.49941899275595225No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1930.47716765149454843No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1810.4474991964793434No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1660.410413627710337No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1640.4054688852078028No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1560.3856899151976661No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1450.3584938314337281No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1310.32388063391598887No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1210.2991569214033179No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT1170.2892674363982496No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1160.2867950651469825No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1160.2867950651469825No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1130.2793779513931812No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1120.2769055801419141No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.2645437238855787No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1050.2595989813830445No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG980.24229238262417485No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG950.2348752688703736No Hit
GACTACCCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT920.22745815511657233No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG910.2249857838653052No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT900.22251341261403812No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT900.22251341261403812No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG860.21262392760896978No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT810.20026207135263432No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT810.20026207135263432No Hit
GACTACCAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT770.19037258634756596No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT760.18790021509629887No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG690.17059361633742923No Hit
GACTACTGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT680.16812124508616214No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG680.16812124508616214No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT680.16812124508616214No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG680.16812124508616214No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG670.16564887383489504No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG670.16564887383489504No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG660.16317650258362795No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG660.16317650258362795No Hit
GACTACTAGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT660.16317650258362795No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG640.1582317600810938No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT620.1532870175785596No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG620.1532870175785596No Hit
GACTACTCGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT620.1532870175785596No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG610.15081464632729252No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT600.14834227507602543No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT600.14834227507602543No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG600.14834227507602543No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG590.1458699038247583No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG590.1458699038247583No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG590.1458699038247583No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG580.14339753257349125No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG570.14092516132222416No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG570.14092516132222416No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG570.14092516132222416No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG560.13845279007095704No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG560.13845279007095704No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG560.13845279007095704No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT560.13845279007095704No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG560.13845279007095704No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT550.13598041881968997No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCACCCGTAGGCTGTCTCT540.13350804756842288No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT530.1310356763171558No Hit
GACTACTGGGGTATCTAATCCACATCTGCAGCCGCCCGTAGGCTGTCTCT530.1310356763171558No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG520.1285633050658887No Hit
GACTACAAGGGTATCTAATCCACATCTGCAGCCGCCCGTAGGCTGTCTCT520.1285633050658887No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG520.1285633050658887No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT510.1260909338146216No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCT510.1260909338146216No Hit
GACTACCAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCT510.1260909338146216No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG500.1236185625633545No Hit
GACTACACGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT500.1236185625633545No Hit
GACTACAAGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCT500.1236185625633545No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG500.1236185625633545No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT500.1236185625633545No Hit
GACTACACGGGTATCTAATCCTCGTCTGCTGCCTCCCGTAGGCTGTCTCT500.1236185625633545No Hit
GACTACCAGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT490.12114619131208743No Hit
GACTACCCGGGTATCTAATCCACATCTGCAGCCCCCCGTAGGCTGTCTCT490.12114619131208743No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG490.12114619131208743No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCAG490.12114619131208743No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG490.12114619131208743No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG490.12114619131208743No Hit
GACTACCCGGGTATCTAATCCACATCTGCAGCCGCCCGTAGGCTGTCTCT490.12114619131208743No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG490.12114619131208743No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG480.11867382006082033No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG480.11867382006082033No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT480.11867382006082033No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG480.11867382006082033No Hit
GACTACCCGGGTATCTAATCCACATCTGCAGCCTCCCGTAGGCTGTCTCT470.11620144880955324No Hit
GACTACAAGGGTATCTAATCCACATCTGCAGCCCCCCGTAGGCTGTCTCT470.11620144880955324No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT470.11620144880955324No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG470.11620144880955324No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG460.11372907755828617No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG460.11372907755828617No Hit
GACTACCCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCT460.11372907755828617No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT460.11372907755828617No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCATGCTTTCGTACCTCAG460.11372907755828617No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG460.11372907755828617No Hit
GACTACTGGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCTGCCT460.11372907755828617No Hit
GACTACAGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT460.11372907755828617No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG460.11372907755828617No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT450.11125670630701906No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG450.11125670630701906No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT450.11125670630701906No Hit
GACTACCCGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT450.11125670630701906No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAG450.11125670630701906No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG440.10878433505575197No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG440.10878433505575197No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG440.10878433505575197No Hit
GACTACCGGGGTATCTAATCCACATCTGCTGCCACCCGTAGGCTGTCTCT430.10631196380448489No Hit
GACTACCGGGGTATCTAATCCACATCTGCAGCCACCCGTAGGCTGTCTCT430.10631196380448489No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCACCCGTAGGCTGTCTCT430.10631196380448489No Hit
GACTACCAGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT430.10631196380448489No Hit
GACTACTCGGGTATCTAATCCACATCTGCTGCCGCCCGTAGGCTGTCTCT430.10631196380448489No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG430.10631196380448489No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG430.10631196380448489No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG430.10631196380448489No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCTCCCGTAGGCTGTCTCT430.10631196380448489No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCCCCCGTAGGCTGTCTCT420.10383959255321779No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG420.10383959255321779No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG420.10383959255321779No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG420.10383959255321779No Hit
GACTACTCGGGTATCTAATCCACATCTGCAGCCCCCCGTAGGCTGTCTCT420.10383959255321779No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT420.10383959255321779No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCAGCCCCCCGTAGGCTGTCTCT420.10383959255321779No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCGCCCGTAGGCTGTCTCT420.10383959255321779No Hit
GACTACCCGGGTATCTAATCCTCGTCTGCAGCCGCCCGTAGGCTGTCTCT420.10383959255321779No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCAG420.10383959255321779No Hit
GACTACAAGGGTATCTAATCCACATCTGCTGCCCCCCGTAGGCTGTCTCT420.10383959255321779No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT420.10383959255321779No Hit
GACTACAGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT410.1013672213019507No Hit
GACTACCGGGGTATCTAATCCACATCTGCTGCCTCCCGTAGGCTGTCTCT410.1013672213019507No Hit
GACTACCAGGGTATCTAATCCACATCTGCTGCCCCCCGTAGGCTGTCTCT410.1013672213019507No Hit
GACTACCGGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT410.1013672213019507No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG410.1013672213019507No Hit
GACTACCCGGGTATCTAATCCACATCTGCTGCCCCCCGTAGGCTGTCTCT410.1013672213019507No Hit
GACTACCAGGGTATCTAATCCACATCTGCTGCCGCCCGTAGGCTGTCTCT410.1013672213019507No Hit
GACTACAAGGGTATCTAATCCTCGTCTGCTGCCGCCCGTAGGCTGTCTCT410.1013672213019507No Hit
GACTACCGGGGTATCTAATCCACATCTGCTGCCGCCCGTAGGCTGTCTCT410.1013672213019507No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)