FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008119770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45016
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG31056.8975475386529235No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG28646.3621823351697175No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG23665.255909010129732No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG23045.118180202594632No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG20034.449529056335525No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG18794.174071441265328No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG18214.045228363248623No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG15263.3899058112671048No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG13823.0700195486049404No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7301.6216456371068066No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6601.466145370534921No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5341.186244890705527No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5221.1595877021503465No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4490.9974231384396659No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4270.9485516260885017No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4270.9485516260885017No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3430.7619513062022392No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2900.6442153900835259No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1720.38208636929091877No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1500.3332148569397547No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1380.3065576683845744No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1210.26879331793140215No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1180.262129020792607No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1140.2532432912742136No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1140.2532432912742136No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG1130.25102185889461526No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1110.2465789941354185No Hit
GACTACTGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1080.2399146969966234No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1060.2354718322374267No Hit
GACTACTCGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG990.21992180558023816No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC920.20437177892304956No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG910.2021503465434512No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC860.19104318464545939No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA820.18215745512706594No Hit
GACTACCCGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG810.17993602274746756No Hit
GACTACCGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG800.17771459036786919No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGCACATCAG800.17771459036786919No Hit
GACTACTAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG780.17327172560867246No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG780.17327172560867246No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC780.17327172560867246No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA770.1710502932290741No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG770.1710502932290741No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC730.16216456371068064No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA720.15994313133108226No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC720.15994313133108226No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG710.1577216989514839No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG660.1466145370534921No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGCACATCAG660.1466145370534921No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGCACATCAG650.1443931046738937No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG640.14217167229429536No Hit
GACTACAGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG620.1377288075350986No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG620.1377288075350986No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA600.1332859427759019No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG600.1332859427759019No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA590.1310645103963035No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG580.12884307801670516No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG560.12440021325750845No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGCACATCAG560.12440021325750845No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA540.1199573484983117No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGCACATCAG530.11773591611871335No Hit
GACTACCAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG520.11551448373911498No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG510.11329305135951663No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGCACATCAG500.11107161897991825No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG490.10885018660031988No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG480.10662875422072153No Hit
GACTACAAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG480.10662875422072153No Hit
GACTACACGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG480.10662875422072153No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA480.10662875422072153No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG460.10218588946152478No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)