FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008119797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41236
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT34368.33252497817441No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT26806.499175477737899No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT23635.7304297216024835No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT22835.536424483461054No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT21325.170239596469105No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT15723.8122029294790956No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT12883.12348433407702No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT8842.1437578814627996No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2440.591715976331361No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2220.5383645358424677No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT2060.4995634882141818No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1780.43166165486468133No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1730.41953632748084196No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1550.37588514889902025No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1490.36133475603841303No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1490.36133475603841303No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1450.35163449413134157No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1450.35163449413134157No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1350.32738383936366283No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1310.3176835774565913No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1260.30555825007275195No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1250.3031331845959841No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1250.3031331845959841No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC1210.2934329226889126No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1190.2885827917353769No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1160.28130759530507327No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1030.2497817441070909No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT990.24008148220001937No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT990.24008148220001937No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT960.23280628576971577No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC930.22553108933941216No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC930.22553108933941216No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT920.22310602386264428No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC920.22310602386264428No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT910.22068095838587645No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT900.21825589290910855No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC890.21583082743234067No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT870.21098069647880494No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC830.20128043457173345No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT820.19885536909496557No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT800.1940052381414298No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC790.19158017266466193No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC760.18430497623435832No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT750.18187991075759047No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC730.1770297798040547No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC730.1770297798040547No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT720.17460471432728683No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC700.16975458337375107No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT680.16490445242021534No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT680.16490445242021534No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT660.16005432146667958No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT650.15762925598991173No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT640.15520419051314385No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT610.14792899408284024No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT610.14792899408284024No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC600.14550392860607236No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT600.14550392860607236No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT600.14550392860607236No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT540.13095353574546512No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT530.12852847026869724No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC530.12852847026869724No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT520.12610340479192939No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT520.12610340479192939No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT520.12610340479192939No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT510.12367833931516152No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT500.12125327383839364No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC480.11640314288485788No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT470.11397807740809003No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT470.11397807740809003No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT460.11155301193132214No Hit
ACTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT450.10912794645455427No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGAAAGCCTGAT440.1067028809777864No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT430.10427781550101853No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTAGGAT430.10427781550101853No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGAGGAT420.10185275002425065No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)