FastQCFastQC Report
Wed 8 May 2024
EGAF00008119816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128529
Total Bases38.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG58364.540609512250153No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG57794.496261544087327No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG48073.740011981731749No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG47493.69488597903975No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG46253.5984096974223716No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG42193.2825276785783752No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG41183.203946191131962No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG38663.0078814897805164No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG38492.994654902784586No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG12530.974877265053023No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG12250.9530922982361957No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10670.8301628426269558No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG10220.7951512888141976No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9990.7772564946432322No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9830.7648079421764739No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9370.7290183538345432No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9230.7181258704261295No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA8940.6955628690801298No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA8760.6815582475550265No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8460.6582172116798544No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8030.624761726925441No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8000.6224276233379238No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7980.620871554279579No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7930.616981381633717No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7570.5889721385835103No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7370.5734114480000623No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG7340.5710773444125451No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7330.5702993098833726No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6870.5345097215414419No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6750.5251733071913731No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6680.5197270654871663No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6550.509612616607925No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6400.497942098670339No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6290.48938371884944254No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG6070.4722669592076496No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6050.47071089014930484No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5990.4660426829742704No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA5890.4582623376825463No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5780.4497039578616499No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG5710.44425771615744303No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5570.4333652327490294No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5530.4302530946323398No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG5520.4294750601031674No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA5330.4146924040488917No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA5260.4092461623446848No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5130.3991317134654436No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5040.392129402702892No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG4890.38045888476530587No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4810.37423460853192664No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4800.3734565740027542No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4670.363342125123513No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4400.34233519283585806No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG4280.33299877848578924No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4120.3205502260190307No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3970.30887970808144466No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3950.30732363902309984No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3840.29876525920220337No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3800.2956531210855138No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3790.29487508655634137No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3740.29098491391047937No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3710.28865081032296214No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3700.2878727757937897No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3650.2839826031479277No Hit
GACTACAAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3630.2824265340895829No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3530.27464618879785885No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3480.27075601615199685No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3450.2684219125644796No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3450.2684219125644796No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3370.2621976363311004No Hit
GACTACACGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG3310.2575294291560659No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3210.2497490838643419No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3190.2481930148059971No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3160.2458589112184799No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3150.24508087668930748No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3020.23496642781006624No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG3010.2341883932808938No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2890.22485197893082495No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2860.22251787534330772No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2850.22173984081413534No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2840.22096180628496292No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2820.2194057372266181No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2800.2178496681682733No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2780.2162935991099285No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2710.21084735740572166No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2630.2046230811723424No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2560.19917683946813558No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2560.19917683946813558No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2550.1983988049389632No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2470.19217452870558394No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2420.18828435605972194No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2420.18828435605972194No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2380.18517221794303232No Hit
GACTACTTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2350.1828381143555151No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2330.1812820452971703No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2320.18050401076799788No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2300.17894794170965309No Hit
GACTACCTGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2300.17894794170965309No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2260.17583580359296344No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2240.17427973453461865No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2230.17350170000544624No Hit
GACTACATGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2210.17194563094710144No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2120.1649433201845498No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2080.16183118206786018No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2070.16105314753868777No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2050.15949707848034295No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2040.15871904395117056No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG2030.15794100942199815No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2000.15560690583448095No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1960.1524947677177913No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1960.1524947677177913No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1960.1524947677177913No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1900.1478265605427569No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1860.14471442242606727No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1820.14160228430937766No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1720.1338219390176536No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1720.1338219390176536No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1720.1338219390176536No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1700.13226586995930878No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1700.13226586995930878No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1690.1314878354301364No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1650.12837569731344678No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1650.12837569731344678No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1640.12759766278427437No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1640.12759766278427437No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1620.12604159372592955No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1610.12526355919675716No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1490.11592714484668828No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1470.11437107578834349No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACACTTTCGAGCCTCAG1300.10114448879241261No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)