FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008119823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69011
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT29604.289171291533234No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17802.579298952340931No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17402.5213371781310228No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT17292.505397690223298No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT16382.3735346538957556No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT16162.341655678080306No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT13021.8866557505325239No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT10211.4794742867079163No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC5400.7824839518337656No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3200.46369419367926856No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT3190.4622451493240208No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3150.45644897190302997No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3130.4535508831925345No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3130.4535508831925345No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2990.43326426221906655No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC2510.3637101331671762No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2440.35356682268044226No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT2060.2985031371810291No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT2040.29560504847053365No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT2030.294156004115286No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1960.28401269362855197No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1880.27242033878657024No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1860.26952225007607483No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1800.26082798394458856No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1780.2579298952340931No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC1770.2564808508788454No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1760.2550318065235977No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1730.25068467345785456No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1720.24923562910260685No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1720.24923562910260685No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1640.23764327426062512No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1590.23039805248438655No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC1590.23039805248438655No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1570.22749996377389115No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1500.21735665328715711No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1500.21735665328715711No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1470.21300952022141398No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT1460.21156047586616628No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1460.21156047586616628No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT1460.21156047586616628No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1450.21011143151091854No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1440.20866238715567084No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCGGTAGTCCTGTCTCT1400.20286620973468No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC1390.20141716537943224No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1370.1985190766689368No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT1360.1970700323136891No Hit
CCTACGGGAGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1340.19417194360319368No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1310.18982481053745054No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1310.18982481053745054No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1270.1840286331164597No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGAGTAGTCCTGTCTCT1260.18257958876121197No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1250.18113054440596427No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1170.16953818956398253No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1160.16808914520873483No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTGGTAGTCCTGTCTCT1150.16664010085348713No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT1100.15939487907724856No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1100.15939487907724856No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1090.15794583472200083No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT1090.15794583472200083No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCTAGTAGTCCTGTCTCT1070.15504774601150542No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1070.15504774601150542No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1060.1535987016562577No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT1050.15214965730101No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT1050.15214965730101No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1040.15070061294576229No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT1030.14925156859051456No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT1030.14925156859051456No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT1030.14925156859051456No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT1030.14925156859051456No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC1030.14925156859051456No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT1010.14635347988001912No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT1000.14490443552477142No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT1000.14490443552477142No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT1000.14490443552477142No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT980.14200634681427599No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC970.14055730245902828No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT970.14055730245902828No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT970.14055730245902828No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT970.14055730245902828No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT960.13910825810378055No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT960.13910825810378055No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT950.13765921374853285No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT950.13765921374853285No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT950.13765921374853285No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT940.13621016939328512No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT940.13621016939328512No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT940.13621016939328512No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT940.13621016939328512No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCCTGTAGTCCTGTCTCT930.13476112503803742No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT930.13476112503803742No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC920.13331208068278969No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT900.13041399197229428No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT890.12896494761704655No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT890.12896494761704655No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT880.12751590326179885No Hit
CCTACGGGAGGCAGCAGATGTGGATTAGATACCCGTGTAGTCCTGTCTCT880.12751590326179885No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT880.12751590326179885No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC880.12751590326179885No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC870.12606685890655114No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC860.12461781455130343No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGTGTCAGATGTGTAACCGCGGGAT850.1231687701960557No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT850.1231687701960557No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT840.12171972584080798No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGGATTAGATACCC830.12027068148556028No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT830.12027068148556028No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC830.12027068148556028No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT830.12027068148556028No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT820.11882163713031256No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT820.11882163713031256No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC820.11882163713031256No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC820.11882163713031256No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCCAGTAGTCCTGTCTCT810.11737259277506484No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT810.11737259277506484No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC800.11592354841981714No Hit
CCTACGGGTGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT800.11592354841981714No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT800.11592354841981714No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGGCGCAAGCCTGAT800.11592354841981714No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT790.11447450406456942No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT790.11447450406456942No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT790.11447450406456942No Hit
CCTACGGGCGGCAGCAGATGTGGATTAGATACCCTTGTAGTCCTGTCTCT790.11447450406456942No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT780.11302545970932171No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCCAGTAGTCCTGTCTCT780.11302545970932171No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT780.11302545970932171No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT770.11157641535407398No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGTGGAAGCCTGAT770.11157641535407398No Hit
CCTACGGGCGGCAGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT760.11012737099882627No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCGGGTAGTCCTGTCTCT760.11012737099882627No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCCGGTAGTCCTGTCTCT760.11012737099882627No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT760.11012737099882627No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT750.10867832664357856No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCTGGTAGTCCTGTCTCT740.10722928228833084No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC740.10722928228833084No Hit
CCTACGGGTGGCAGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT730.10578023793308314No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC730.10578023793308314No Hit
CCTACGGGAGGCTGCAGACGAGGATTAGATACCCTTGTAGTCCTGTCTCT730.10578023793308314No Hit
CCTACGGGGGGCTGCAGACGAGGATTAGATACCCCTGTAGTCCTGTCTCT730.10578023793308314No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGAAAGCCTGAT720.10433119357783542No Hit
CCTACGGGCGGCTGCAGATGTGGATTAGATACCCCGGTAGTCCTGTCTCT720.10433119357783542No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCGCAATGGGGGCAACCCTGAC720.10433119357783542No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC720.10433119357783542No Hit
CCTACGGGAGGCAGCAGGATTAGATACCCTTGTAGTCCTGTCTCTTATAC710.1028821492225877No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC710.1028821492225877No Hit
CCTACGGGCGGCAGCAGACGAGGATTAGATACCCGAGTAGTCCTGTCTCT710.1028821492225877No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCGGGTAGTCCTGTCTCT710.1028821492225877No Hit
CCTACGGGCGGCTGCAGACGAGGATTAGATACCCGTGTAGTCCTGTCTCT710.1028821492225877No Hit
CCTACGGGCGGCAGCAGATGTGGATTAGATACCCGAGTAGTCCTGTCTCT700.10143310486734No Hit
CCTACGGGTGGCTGCAGATGTGGATTAGATACCCCGGTAGTCCTGTCTCT700.10143310486734No Hit
CCTACGGGGGGCAGCAGACGAGGATTAGATACCCTAGTAGTCCTGTCTCT700.10143310486734No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)