FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101063
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG35573.519586792396822No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG32373.2029526137161963No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG27402.711180154952851No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG25942.566715810929816No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG20632.041300970681654No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG20622.040311488873277No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG20552.0333851162146384No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG17251.7068561194502438No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG15831.5663497026607167No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14451.429801213104697No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14131.3981377952366345No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10511.0399453806041776No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9810.9706816540177909No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9590.9489130542334979No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8470.8380910916952792No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG8310.8222593827612479No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG8020.7935644103183164No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7210.7134163838397831No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7100.7025320839476366No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6380.631289393744496No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG6030.5966575304513027No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG5870.5808258215172714No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG5090.5036462404638691No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG4780.47297230440418353No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG4630.4581300772785292No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG4300.4254771776020898No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG3670.3631398236743417No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3160.31267625144711714No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2790.27606542453716987No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG2660.2632021610282695No Hit
GANTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2610.25825475198638476No Hit
GANTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2550.252317861136123No Hit
GANTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2330.23054926135183004No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG2240.22164392507643746No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG2050.20284377071727536No Hit
GACTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG1830.1810751709329824No Hit
GANTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1820.18008568912460543No Hit
GANTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1770.17513828008272067No Hit
GACTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG1630.16128553476544333No Hit
GANTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1590.15732760753193553No Hit
GACTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG1530.1513907166816738No Hit
GANTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1500.14842227125654295No Hit
GACTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG1330.13160108051413474No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG1220.12071678062198826No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCAG1220.12071678062198826No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG1190.1177483351968574No Hit
GACTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG1120.11082196253821873No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1080.10686403530471092No Hit
GACTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1070.10587455349633397No Hit
GACTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1040.10290610807120312No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG1040.10290610807120312No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1030.10191662626282616No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTGAG1020.10092714445444921No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)