FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40622
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2080.5120378120230417No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1860.45787996652060464No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1710.420954162768943No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1650.4061838412682783No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1610.3963369602678351No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1530.3766431982669489No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1510.3717197577667274No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1360.33479395401506573No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1300.3200236325144011No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1160.2855595490128502No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1070.2634040667618532No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1020.2510954655112993No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1010.24863374526118853No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG990.24371030476096697No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC990.24371030476096697No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC920.22647826301019156No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG910.22401654276008073No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC900.22155482250996997No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC890.2190931022598592No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG820.20186106050908373No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC710.1747821377578652No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC680.16739697700753287No Hit
NNNTNNTGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC660.1624735365073113No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG650.16001181625720054No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC640.15755009600708977No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC580.1427797745064251No Hit
GANTANTGGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC580.1427797745064251No Hit
NANTANTGGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC560.13785633400620353No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG560.13785633400620353No Hit
NNNTNNTCGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC540.13293289350598197No Hit
NNNTNNTCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC540.13293289350598197No Hit
GNNTANTGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC520.12800945300576042No Hit
GANTANTCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC520.12800945300576042No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC520.12800945300576042No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG510.12554773275564965No Hit
GNNTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC510.12554773275564965No Hit
GNNTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC500.12308601250553887No Hit
GNNTANTCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC500.12308601250553887No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG500.12308601250553887No Hit
GNNTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC490.12062429225542809No Hit
NANTNNCGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC490.12062429225542809No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG480.11816257200531731No Hit
NNNTNNTGGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC480.11816257200531731No Hit
GANTNNCGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC470.11570085175520654No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC470.11570085175520654No Hit
NNNTNNTAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC460.11323913150509578No Hit
GANTNNTGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC450.11077741125498498No Hit
NNCTNNTCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC450.11077741125498498No Hit
NANTANTAGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC450.11077741125498498No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG450.11077741125498498No Hit
GANTANTAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC450.11077741125498498No Hit
NANTANCGGGGTATNTANTNNGAAGACNTTNTTNNTNCACGCGGCGTCGC440.10831569100487422No Hit
NNCTNNCGGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC430.10585397075476342No Hit
NNNTNNCGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC430.10585397075476342No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC430.10585397075476342No Hit
GNNTANCCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC420.10339225050465266No Hit
NNCTNNCCGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC410.10093053025454186No Hit
GANTANCCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC410.10093053025454186No Hit
GNNTANCAGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC410.10093053025454186No Hit
GANTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC410.10093053025454186No Hit
NNNTNNTAGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC410.10093053025454186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)