FastQCFastQC Report
Wed 8 May 2024
EGAF00008119866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74960
Total Bases22.5 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC18602.4813233724653148No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC18022.403948772678762No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13611.8156350053361792No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13121.7502668089647813No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12881.7182497331910351No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11641.5528281750266808No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9871.3167022411953042No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9431.2580042689434365No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8211.0952508004268944No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4600.6136606189967982No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA4470.5963180362860192No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3550.47358591248665954No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3210.4282283884738527No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3170.42289220917822834No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA3060.4082177161152615No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3000.40021344717182494No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2650.35352187833511206No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2570.34284951974386335No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2450.3268409818569904No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2280.30416221985058695No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2210.29482390608324444No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2200.2934898612593383No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2080.2774813233724653No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC2080.2774813233724653No Hit
GACTACTGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA2050.27347918890074707No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2030.2708110992529349No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1900.2534685165421558No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1790.23879402347918888No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1750.23345784418356458No Hit
GACTACCGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1690.22545357524012807No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1680.22411953041622198No Hit
GACTACCGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1560.20811099252934898No Hit
GACTACCCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1560.20811099252934898No Hit
GACTACCCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1540.2054429028815368No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1500.20010672358591247No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1450.19343649946638208No Hit
GACTACCAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1400.18676627534685164No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1370.1827641408751334No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1320.17609391675560299No Hit
GACTACCAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1310.1747598719316969No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1260.1680896478121665No Hit
GACTACTCGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1220.16275346851654215No Hit
GACTACACGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1160.15474919957310568No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1120.14941302027748132No Hit
GACTACAGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1080.144076840981857No Hit
GACTACAGGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1080.144076840981857No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1070.1427427961579509No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1060.14140875133404485No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1050.14007470651013873No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1030.13740661686232658No Hit
GACTACACGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1030.13740661686232658No Hit
GACTACTGGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA1020.1360725720384205No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1010.1347385272145144No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1000.13340448239060831No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA1000.13340448239060831No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG990.13207043756670225No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA960.128068303094984No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG950.1267342582710779No Hit
GACTACAAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA940.1254002134471718No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA930.12406616862326574No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG920.12273212379935966No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA880.11739594450373533No Hit
GACTACAAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC880.11739594450373533No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACACTGCCGACGA870.11606189967982923No Hit
GACTACTGGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA860.11472785485592316No Hit
GACTACTAGGGTATCTAATCCGGCGTGAACACATCTGACGCTGCCGACGA860.11472785485592316No Hit
GACTACTCGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT850.11339381003201707No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG850.11339381003201707No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG840.11205976520811099No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG810.10805763073639274No Hit
GACTACTCGGGTATCTAATCCTCTATGTACACATCTGACGCTGCCGACGA810.10805763073639274No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC810.10805763073639274No Hit
GACTACTCGGGTATCTAATCCACATCTGACGCTGCCGACGACTGCAGCCC780.10405549626467449No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG780.10405549626467449No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG770.1027214514407684No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG770.1027214514407684No Hit
GACTACTAGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT770.1027214514407684No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG760.10138740661686232No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA750.10005336179295624No Hit
GACTACTGGGGTATCTAATCCTCGTCTGCAGCCACCCGTAGGCTGTCTCT750.10005336179295624No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)