FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119882

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119882
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127629
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21111.6540128027329213No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19651.5396187386879157No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15951.249715973642354No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13541.0608874158694341No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13301.042082912190803No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG13061.023278408512172No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG12340.966864897476279No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11770.9222042012395302No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11320.8869457568420971No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG9720.7615823989845568No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG9710.7607988779979472No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9680.7584483150381183No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9590.7513966261586317No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9030.7075194509084926No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8940.700467762029006No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8700.6816632583503749No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8530.6683434015780113No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8350.654240023819038No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7580.5939089078500968No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7410.5805890510777332No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6970.5461141276669096No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6680.5233920190552304No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6540.5124227252426956No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6500.5092886412962571No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6330.4959687845238934No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6040.4732466759122143No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5980.46854554999255654No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG5880.4607103401264603No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5810.45522569322019285No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5750.4505245673005352No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG5560.4356376685549523No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5150.4035133081039576No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG5000.39176049330481316No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4940.38705936738515545No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG4570.35806909088059924No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4360.34161515016179705No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4340.34004810818857784No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4310.33769754522874895No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4030.31575895760367945No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3970.3110578316840217No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3870.3032226218179254No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3700.28990276504556173No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3690.2891192440589521No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3650.2859851601125136No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3580.28050051320624625No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3510.2750158662999788No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG3230.2530772786749093No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3030.23740685894271676No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG3000.2350562959828879No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2880.2256540441435724No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2850.2233034811837435No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2800.2193858762506954No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2660.2084165824381606No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG2520.19744728862562583No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2480.19431320467918733No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2350.1841274318532622No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2280.17864278494699481No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2200.1723746170541178No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG2090.1637558862014119No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2050.16062180225497338No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1920.15043602942904827No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1880.14730194548260975No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1860.1457349035093905No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1850.14495138252278086No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1800.14103377758973276No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1750.1371161726566846No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1710.13398208871024608No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1700.13319856772363647No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1690.13241504673702684No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1620.12693039983075946No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1620.12693039983075946No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1570.12301279489771134No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1550.12144575292449208No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1520.11909518996466321No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1510.11831166897805358No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1410.1104764591119573No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1370.10734237516551881No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1360.10655885417890917No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGAGCCTCAG1330.1042082912190803No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1330.1042082912190803No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1300.10185772825925142No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1300.10185772825925142No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGAGCCTCAG1280.10029068628603216No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)