FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008119884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49308
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11522.3363348746653685No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10812.1923420134663747No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8331.689381033503691No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8101.6427354587490874No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7951.6123144317352154No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6661.3506935994159162No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6181.2533463129715259No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5461.1073253833049403No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4640.9410237689624401No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3670.7443011276060679No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3010.6104486087450313No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2540.5151293907682324No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2450.4968767745599092No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2430.49282063762472617No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2350.4765960898839945No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG2280.4623996106108542No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2250.4563154052080798No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2240.4542873367404884No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1920.38938914577756145No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1910.38736107730997No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1890.3833049403747871No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1870.3792488034396041No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1830.37113652956923826No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1810.36708039263405534No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1810.36708039263405534No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1800.3650523241664638No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1800.3650523241664638No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1790.36302425569887237No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1750.35491198182850653No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1740.35288391336091507No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1740.35288391336091507No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1730.35085584489332355No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1710.34679970795814064No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1710.34679970795814064No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1710.34679970795814064No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1680.34071550255536626No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1670.3386874340877748No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1670.3386874340877748No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1660.33665936562018334No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1660.33665936562018334No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1650.3346312971525919No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1640.33260322868500036No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1600.3244909548146345No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1590.32246288634704307No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1570.31840674941186015No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1550.3143506124766772No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1540.3123225440090857No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1460.29609799626835404No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1410.2859576539303967No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1360.27581731159243933No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1340.2717611746572564No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1340.2717611746572564No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1310.26567696925448203No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1280.25959276385170765No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1270.2575646953841162No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1260.2555366269165247No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1250.2535085584489332No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1230.2494524215137503No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1230.2494524215137503No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1220.24742435304615884No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1220.24742435304615884No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1210.24539628457856738No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG1210.24539628457856738No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1200.24336821611097592No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1200.24336821611097592No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1200.24336821611097592No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1190.24134014764338446No Hit
GANTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1170.2372840107082015No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1160.23525594224061003No Hit
GNNTANCAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1160.23525594224061003No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1160.23525594224061003No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1150.23322787377301857No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1150.23322787377301857No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1150.23322787377301857No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1140.2311998053054271No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG1130.22917173683783565No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1120.2271436683702442No Hit
NNCTNNAGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG1120.2271436683702442No Hit
NNNTNNACGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1100.22308753143506127No Hit
NNNTNNACGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1080.21903139449987835No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1080.21903139449987835No Hit
NANTANACGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1070.21700332603228684No Hit
GANTANAGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1070.21700332603228684No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1070.21700332603228684No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1070.21700332603228684No Hit
GNNTANACGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG1060.21497525756469538No Hit
NNNTNNAGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG1050.21294718909710392No Hit
NANTANAGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG1050.21294718909710392No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1030.208891052161921No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG1030.208891052161921No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1020.20686298369432954No Hit
GANTANACGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG1000.2028068467591466No Hit
GNNTANAGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG990.20077877829155513No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG990.20077877829155513No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG960.19469457288878073No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG940.1906384359535978No Hit
NNNTNNAAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG940.1906384359535978No Hit
GANTNNAGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG920.18658229901841486No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG910.1845542305508234No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG900.1825261620832319No Hit
NANTANAAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG890.18049809361564045No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG890.18049809361564045No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA890.18049809361564045No Hit
NANTNNACGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG880.178470025148049No Hit
GANTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG860.17441388821286605No Hit
GNNTANAAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG850.1723858197452746No Hit
GANTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG840.17035775127768313No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG840.17035775127768313No Hit
GANTANAAGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG830.16832968281009167No Hit
NANTNNAAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG820.16630161434250018No Hit
NNNTNNCAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG820.16630161434250018No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG780.15818934047213434No Hit
GANTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG770.15616127200454286No Hit
GANTNNACGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG760.1541332035369514No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG700.1419647927314026No Hit
NNNTNNAAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG690.13993672426381115No Hit
GANTNNAAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG690.13993672426381115No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG680.13790865579621966No Hit
GANTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG650.13182445039344529No Hit
NNCTNNAAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG610.12371217652307942No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA590.1196560395878965No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG560.1135718341851221No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA550.11154376571753064No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA520.10545956031475623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)