FastQCFastQC Report
Wed 8 May 2024
EGAF00008119889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77754
Total Bases23.4 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT20432.6275175553669263No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC16352.1027857087738253No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT15021.9317334156442112No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT14341.8442781078786945No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT13661.7568228001131774No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC13461.7311006507703781No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT12521.6102065488592228No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC12221.571623324845024No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT11181.4378681482624687No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC10501.3504128404969518No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT9861.2681019625999947No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC9751.2539547804614553No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC9311.1973660519072975No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC7550.9710111376906654No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7450.9581500630192659No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7410.953005633150706No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7180.9234251614064871No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT7100.9131363016693674No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6970.8964169045965481No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6860.8822697224580086No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6460.8308254237724103No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC6220.7999588445610516No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6200.7973866296267716No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGCAACCCTGAC6090.783239447488232No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC6060.7793811250868122No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5340.6867813874527355No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC5230.672634205314196No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4930.6340509812999974No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4880.6276204439642976No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4380.5633150706072999No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC4130.5311623839288011No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC4120.5298762764616611No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3510.451423720966124No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3440.4424209686961443No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC3230.41541271188620515No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC3170.40769606708336553No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC3140.40383774468194567No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2970.3819739177405664No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2860.3678267356020269No Hit
CCTACGGGGGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2700.34724901612778764No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2690.34596290866064766No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2640.3395323713249479No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2590.33310183398924814No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT2550.32795740412068836No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT2470.3176685443835687No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2350.30223525477788926No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2320.2983769323764694No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCCGCAATGGGCGAAAGCCTGAC2220.2855158577050698No Hit
CCTACGGGTGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2220.2855158577050698No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT2210.2842297502379299No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT2080.2675103531651105No Hit
CCTACGGGCGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC2060.26493813823083057No Hit
CCTACGGGTGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1830.2353576664866116No Hit
CCTACGGGGGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1820.23407155901947166No Hit
CCTACGGGGGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1790.2302132366180518No Hit
CCTACGGGAGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1770.22764102168377187No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1760.22635491421663195No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1750.225068806749492No Hit
CCTACGGGCGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1710.2199243768809322No Hit
CCTACGGGCGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1660.2134938395452324No Hit
CCTACGGGGGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1620.20834940967667256No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1600.20577719474239264No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1560.20063276487383286No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1470.18905779766957326No Hit
CCTACGGGGGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1450.18648558273529336No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1380.17748283046531368No Hit
CCTACGGGAGGCAGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT1370.17619672299817374No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1360.17491061553103376No Hit
CCTACGGGCGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1220.1569051109910744No Hit
CCTACGGGAGGCTGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1220.1569051109910744No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1200.1543328960567945No Hit
CCTACGGGCGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT1170.15047457365537464No Hit
CCTACGGGAGGCAGCAGTAGGGAATCTTCGGCAATGGGGGGAACCCTGAC1150.14790235872109472No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1140.14661625125395478No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTAGACTTGGGAT1130.14533014378681483No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1120.14404403631967488No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT1080.13889960645111504No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1020.13118296164827534No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1020.13118296164827534No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT1010.1298968541811354No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC1000.12861074671399542No Hit
CCTACGGGAGGCTGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT960.12346631684543562No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT940.1208941019111557No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT920.11832188697687579No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT900.11574967204259587No Hit
CCTACGGGTGGCAGCAGTAGGGAATCTTCCGCAATGGACGCAAGTCTGAC880.11317745710831598No Hit
CCTACGGGAGGCAGCAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGAT840.10803302723975615No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT840.10803302723975615No Hit
CCTACGGGCGGCTGCAGTCGTCGGCAGTGTCAGATGTGTACATAGCGGAT790.10160248990405639No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)