FastQCFastQC Report
Wed 8 May 2024
EGAF00008119894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73931
Total Bases22.2 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG8541.1551311357887761No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG8091.0942635700856205No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG6530.8832560089813474No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG6380.8629668204136289No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG5850.7912783541410234No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG5010.6776588981617995No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4450.6019125941756502No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4370.5910916936062004No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3610.4882931381964264No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3560.48153007534052017No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3310.44771476106098934No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG3100.4193098970661833No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2850.38549458278665244No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2640.3570897187918465No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2510.33950575536649036No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2490.3368005302241279No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2380.3219217919411343No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2350.3178639542275906No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2110.2854012525192409No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2050.27728557709215346No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2030.274580351949791No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1910.25834900109561615No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1900.25699638852443496No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1820.24617548795498506No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1780.24076503767026008No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1770.23941242509907887No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1740.23535458738553516No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1680.22723891195844775No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1590.2150653988178166No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1590.2150653988178166No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1550.20965494853309166No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1510.20424449824836674No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1450.1961288228212793No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1440.19477621025009806No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1370.1853079222518294No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1340.1812500845382857No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG1300.17583963425356075No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1270.17178179654001705No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1220.16501873368411085No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1200.1623135085417484No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1190.16096089597056715No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1180.15960828339938593No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG1160.15690305825702344No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1150.1555504456858422No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1140.15419783311466098No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG1090.1474347702587548No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1090.1474347702587548No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1080.14608215768757354No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG1070.14472954511639233No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1050.14202431997402984No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1040.1406717074028486No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1020.13796648226048613No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG1010.1366138696893049No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1010.1366138696893049No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1010.1366138696893049No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1000.13526125711812367No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG990.1339086445469424No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG980.13255603197576118No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG970.13120341940457994No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG970.13120341940457994No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG970.13120341940457994No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG940.12714558169103624No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG940.12714558169103624No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG930.12579296911985502No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG920.12444035654867376No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG920.12444035654867376No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG890.12038251883513004No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG860.11632468112158635No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG860.11632468112158635No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG850.1149720685504051No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG840.11361945597922388No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG840.11361945597922388No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG830.11226684340804263No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG830.11226684340804263No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG830.11226684340804263No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG820.11091423083686139No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG820.11091423083686139No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG810.10956161826568016No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG810.10956161826568016No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG800.10820900569449893No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG800.10820900569449893No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG800.10820900569449893No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG800.10820900569449893No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC790.1068563931233177No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG790.1068563931233177No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG780.10550378055213644No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG780.10550378055213644No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG760.10279855540977398No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG750.10144594283859273No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG750.10144594283859273No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG750.10144594283859273No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG740.1000933302674115No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG740.1000933302674115No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG740.1000933302674115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)