FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82294
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC19112.322162004520378No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC17862.170267577223127No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC14801.7984300191994558No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13981.6987872748924586No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12571.5274503609011594No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12011.4594016574719906No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8951.0875640994483193No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8891.0802731669380514No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8261.0037183755802366No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5430.6598293921792597No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5110.6209444187911634No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG5020.6100080200257614No Hit
GANTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4680.5686927358009088No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG4510.5480350936884828No Hit
GANTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3870.47026514691229004No Hit
GANTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3860.4690499914939121No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG3710.4508226602182419No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG3470.42165893017716966No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG3310.4022164434831215No Hit
GANTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3280.3985709772279874No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG2980.3621163146766472No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG2750.3341677400539529No Hit
GANTANACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2700.32809196296206283No Hit
GANTANAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2470.3001433883393686No Hit
GANTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2450.2977130775026126No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG2110.25639779327776024No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG2110.25639779327776024No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1930.23452499574695604No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCACCTCAG1530.18591877901183562No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCACCTCAG1510.1834884681750796No Hit
GANTANTGGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG1500.18227331275670158No Hit
GACTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1410.17133691399129947No Hit
GACTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1370.16647629231778743No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1350.16404598148103142No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1350.16404598148103142No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1300.15797020438914136No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1290.15675504897076337No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1270.15432473813400735No Hit
GACTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1260.15310958271562933No Hit
GANTANTCGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG1180.14338833936860523No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCGCACCTTCGTATATCAG1170.1421731839502272No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCACCTCAG1170.1421731839502272No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1170.1421731839502272No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1130.13731256227671518No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1110.13488225143995916No Hit
GANTANCGGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG1110.13488225143995916No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1070.13002162976644713No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCGCACCTTCGTATATCAG1050.1275913189296911No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCACCTCAG1050.1275913189296911No Hit
GACTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1020.12394585267455707No Hit
GANTANCAGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG990.12030038641942305No Hit
GANTANTAGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG990.12030038641942305No Hit
GANTANCCGGGTATCTAATCCTGTTTGATACCCACGCCTTCGTGCTTCAG970.11787007558266703No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA960.11665492016428901No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA960.11665492016428901No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCGCACCTTCGTATATCAG960.11665492016428901No Hit
GACTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC920.11179429849077697No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC920.11179429849077697No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC910.11057914307239895No Hit
GACTACAGGGGTATCTAATCCCGTTCGCTACCCTGGCTTTCGCACCTCAG880.10693367681726493No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC880.10693367681726493No Hit
GANTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC880.10693367681726493No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA870.10571852139888692No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC870.10571852139888692No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG860.10450336598050891No Hit
GACTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC860.10450336598050891No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG860.10450336598050891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)