FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177454
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG155278.74987320657748No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG140207.900639038849505No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG114736.465337495914435No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG110756.241054019633257No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG92615.218817270954727No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG85744.831674687524655No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG85284.805752476698187No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG73254.127830310953825No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG65223.675318674135269No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18821.060556538595918No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18281.030126117190934No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15260.8599411678519504No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG14520.8182402200006763No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11630.6553811128517814No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11310.6373482705377168No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11290.6362212178930878No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9200.5184442165293541No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8370.4716715317772493No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7580.4271529523144026No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6500.36629210950443497No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5400.30430421404983826No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4500.2535868450415319No Hit
GACTACTGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG4370.24626100285144317No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4270.24062573962829806No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG4260.2400622133059835No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4220.23780810801672544No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4190.2361175290497819No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3980.22428347628117706No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG3720.20963179190099968No Hit
GACTACTCGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG3690.20794121293405612No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3610.20343300235554002No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG3390.1910354232646207No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3190.17976489681833038No Hit
GACTACCGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG3150.17751079152907231No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG3140.1769472652067578No Hit
GACTACTAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG3120.1758202125621288No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3070.1730025809505562No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG3020.17018494933898362No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2930.165113212438153No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2920.16454968611583845No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG2770.15609679128112075No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2730.15384268599186268No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2710.15271563334723365No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG2670.15046152805797558No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG2570.14482626483483044No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG2550.1436992121902014No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2470.13919100161168527No Hit
GACTACCAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG2430.13693689632242723No Hit
GACTACCCGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG2430.13693689632242723No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2430.13693689632242723No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG2400.13524631735548368No Hit
GACTACACGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG2320.13073810677696757No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG2160.12172168561993531No Hit
GACTACAGGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG2160.12172168561993531No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2160.12172168561993531No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2060.11608642239679015No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG2050.11552289607447565No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG2050.11552289607447565No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG2040.11495936975216112No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2020.1138323171075321No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1980.11157821181827403No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1970.11101468549595951No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1950.10988763285133048No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1940.10932410652901597No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATCAG1940.10932410652901597No Hit
GACTACAAGGGTATCTAATCNTGTTTGATCCCCACGCTTTCGCACATCAG1910.10763352756207242No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGNTTTCGCACATCAG1820.10256179066124178No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)