FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008119946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125553
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG35392.8187299387509657No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG31532.511290052806385No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG28232.2484528446154215No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG26352.098715283585418No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25482.0294218377896187No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25232.009509928078182No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG21521.7140171879604629No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG20831.659060317156898No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20821.6582638407684405No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG20571.6383519310570038No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG19221.5308276186152463No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG18401.4655165547617341No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17111.3627711006507213No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG16481.312593088177901No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG16441.3094071826240712No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG15291.2178123979514626No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15221.2122370632322603No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG14021.1166598966173649No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG13901.1071021799558753No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG13101.0433840688792781No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG11870.9454174730990099No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG11800.9398421383798077No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG11060.8809028856339554No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG10740.8554156412033165No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9760.7773609551344851No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9620.7662102856960806No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG9550.7606349509768783No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA9080.7232005607193775No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA8910.7096604621156006No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8480.6754119774119296No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8090.6443493982620886No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8090.6443493982620886No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7440.5925784330123534No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA7310.5822242399624064No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7240.5766489052432041No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7190.5726665233009167No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA7160.5702770941355444No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA7110.5662947121932571No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA6620.5272673691588413No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6100.4858505969590531No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6060.4826646914052233No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA5940.4731069747437337No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA5820.4635492580822441No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA5630.4484162067015523No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5590.4452303011477225No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA5510.4388584900400627No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG5330.42452191504782844No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA5130.40859238727867914No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4960.3950522886749022No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG4910.39106990673261494No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA4770.3799192372942104No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4220.3361130359290499No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGGGCATGAA4170.33213065398676256No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4160.3313341775983051No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3920.312218744275326No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3780.30106807483692144No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3500.2787667359601125No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3470.27637730679474004No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3370.2684125429101654No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3320.2644301609678781No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3300.2628372081909632No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3150.2508900623641012No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG3110.24770415681027136No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3090.24611120403335643No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2670.21265919571814293No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2570.2046944318335683No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2540.2023050026681959No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2490.19832262072590856No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2280.18159661656830184No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2250.17920718740292943No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2230.17761423462601453No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2180.1736318526837272No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2020.16088823046840778No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1960.156109372137663No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1950.15531289574920554No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1930.15371994297229058No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1910.15212699019537565No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1810.14416222631080103No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1760.1401798443685137No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1750.13938336798005624No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1730.1377904152031413No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1650.1314186040954816No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1650.1314186040954816No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1470.11708202910324723No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1470.11708202910324723No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1450.1154890763263323No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1390.11071021799558753No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1330.10593135966484274No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1320.10513488327638527No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1310.1043384068879278No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1300.10354193049947034No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1300.10354193049947034No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1280.1019489777225554No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCACGCTTTCGAGCCTCAG1260.10035602494564048No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)