FastQCFastQC Report
Wed 8 May 2024
EGAF00008119965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52493
Total Bases15.8 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGGGTGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA17903.409978473320252No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA13442.5603413788505134No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA12112.306974263235098No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA11362.1640980702188863No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT10562.0116967976682605No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA9491.8078600956317987No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA7281.3868515802106949No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA7191.3697064370487493No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT7021.3373211666317415No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6301.200160021336178No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA6261.1925399577086468No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT6011.1449145600365762No Hit
CCTACGGGGGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA5661.0782390032956775No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT5461.0401386851580212No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4590.8744023012592155No Hit
CCTACGGGTGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4240.8077267445183167No Hit
CCTACGGGCGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA4220.8039167127045511No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT4190.7982016649839027No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGGACAATGGACCGAGAGTCTGA4100.7810565218219572No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT3500.6667555674089879No Hit
CCTACGGGCGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA3130.5962699788543234No Hit
CCTACGGGTGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2990.5695997561579639No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT2840.5410245175547216No Hit
CCTACGGGAGGCAGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA2640.5029241994170651No Hit
CCTACGGGGGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2260.4305335949555179No Hit
CCTACGGGGGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA2140.40767340407292396No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGAAACCCTGAT2120.40386337225915836No Hit
CCTACGGGTGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1990.37909816546968167No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1760.33528279961137675No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1750.33337778370449395No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1740.3314727677976111No Hit
CCTACGGGAGGCTGCAGTGAGGAATATTGGTCAATGGGCGAGAGCCTGAA1590.30289752919436874No Hit
CCTACGGGCGGCTGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1560.29718248147372034No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1390.26479721105671233No Hit
CCTACGGGCGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1150.21907682929152458No Hit
CCTACGGGAGGCAGCAGTAGGGAATATTGCTCAATGGGGGAAACCCTGAA1120.21336178157087612No Hit
CCTACGGGCGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT1120.21336178157087612No Hit
CCTACGGGTGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT1120.21336178157087612No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC920.17526146343321966No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC910.17335644752633686No Hit
CCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT900.17145143161945403No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT820.15621130436439143No Hit
CCTACGGGGGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC790.15049625664374297No Hit
CCTACGGGTGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT770.14668622482997734No Hit
CCTACGGGAGGCTGCAGTGGGGAATATTGCACAATGGGGGGAACCCTGAT760.1447812089230945No Hit
CCTACGGGTGGCTGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA750.14287619301621168No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT740.14097117710932888No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC740.14097117710932888No Hit
CCTACGGGTGGCTGCAGAATCAATTAGATACCCTGGTAGTCCGGCTGGCT730.13906616120244605No Hit
CCTACGGGTGGCTGCAGTAGGGAATCTTCGGCAATGGACGGAAGTCTGAC650.12382603394738347No Hit
CCTACGGGTGGCTGCAGTAGCGAAGGCGGCTTTCTGGACTGTAACTGACG590.11239593850608652No Hit
CCTACGGGCGGCTGCAGTGGGGAATATTGGGCAATGGAGGAAACTCTGAC590.11239593850608652No Hit
CCTACGGGGGGCTGCAGTCGTCGGCAGCGTCAGATGTGTACATAGCGGAT590.11239593850608652No Hit
CCTACGGGGGGCTGCAGTGGGGGATATTGCACAATGGGGGAAACCCTGAT550.10477587487855525No Hit
CCTACGGGGGGCAGCAGTGAGGAATATTGGTCAATGGGCGGAAGCCTGAA550.10477587487855525No Hit
CCTACGGGTGGCTGCAGTGGGGAATTTTGCACAATGGGGGGAACCCTGAT540.10287085897167242No Hit
CCTACGGGTGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGAT530.10096584306478959No Hit
CCTACGGGGGGCTGCAGTGGGGAATATTGCACAATGGGCGAAAGCCTGAT530.10096584306478959No Hit
CCTACGGGTGGCTGCAGTGGGGAATATTGCACAATGGGCGGAAGCCTGAT530.10096584306478959No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)