FastQCFastQC Report
Wed 9 Aug 2023
EGAF00008119966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52493
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG5381.0248985579029586No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG5341.0172784942754272No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3840.7315261082430038No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3820.7277160764292382No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3700.7048558855466444No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3440.6553254719676909No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3430.6534204560608081No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2820.537214485740956No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2740.5219743584858934No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2420.461013849465643No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2180.4152934677004553No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2180.4152934677004553No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2170.4133884517935725No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2080.39624330863162704No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1990.37909816546968167No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1960.3733831177490332No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1690.321947688263197No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1680.3200426723563142No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1640.31242260872878286No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1600.3048025451012516No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1560.29718248147372034No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1440.27432229059112645No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1350.257177147429181No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1340.2552721315222982No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1290.2457470519878841No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1150.21907682929152458No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1090.20764673385022764No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1070.20383670203646198No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1050.20002667022269638No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG980.1866915588745166No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG900.17145143161945403No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG900.17145143161945403No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC870.16573638389880554No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG860.16383136799192272No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG850.16192635208503992No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG820.15621130436439143No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC820.15621130436439143No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG810.1543062884575086No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG780.14859124073686017No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG770.14668622482997734No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC760.1447812089230945No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG760.1447812089230945No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG760.1447812089230945No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG750.14287619301621168No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG750.14287619301621168No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG750.14287619301621168No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG740.14097117710932888No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG730.13906616120244605No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG720.13716114529556322No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG710.1352561293886804No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG700.13335111348179757No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG680.12954108166803194No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG670.1276360657611491No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG670.1276360657611491No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG660.12573104985426628No Hit
GNNTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC650.12382603394738347No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG650.12382603394738347No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG640.12192101804050064No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG640.12192101804050064No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG630.12001600213361781No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG630.12001600213361781No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC630.12001600213361781No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG630.12001600213361781No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG610.11620597031985218No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG600.11430095441296935No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG600.11430095441296935No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG590.11239593850608652No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG580.1104909225992037No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG580.1104909225992037No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG580.1104909225992037No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG570.10858590669232088No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC570.10858590669232088No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG560.10668089078543806No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG560.10668089078543806No Hit
GANTNNTGGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC550.10477587487855525No Hit
NANTNNCGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC550.10477587487855525No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG550.10477587487855525No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG540.10287085897167242No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG540.10287085897167242No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG540.10287085897167242No Hit
GANTANTCGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC530.10096584306478959No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)