FastQCFastQC Report
Wed 8 May 2024
EGAF00008119972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66211
Total Bases19.9 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG7021.060246786787694No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG6741.0179577411608343No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG5460.8246363897237619No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4960.7491202368186555No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4340.6554802072163236No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3840.5799640543112171No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3710.5603298545558896No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3310.4999169322318044No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2870.43346271767531075No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2430.3670085031188171No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2380.3594568878283065No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2330.3519052725377958No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2300.34737430336348946No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1820.2748787965745873No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1800.271858150458383No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1790.2703478274002809No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1750.2643065351678724No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1660.25071362764495325No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1640.247692981528749No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1610.24316201235444262No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1500.22654845871531917No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1470.2220174895410128No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1360.2054039359018894No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1360.2054039359018894No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1300.19634199755327666No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1260.19030070532086812No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1260.19030070532086812No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1250.188790382262766No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1220.18425941308845964No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1210.1827490900303575No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1210.1827490900303575No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1180.17821812085605113No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1160.17519747473984684No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1150.17368715168174473No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1140.17217682862364259No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1120.16915618250743836No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG1120.16915618250743836No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1100.16613553639123407No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG1090.16462521333313196No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1040.15707359804262133No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1030.1555632749845192No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1020.15405295192641708No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1020.15405295192641708No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG980.14801165969400856No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG980.14801165969400856No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG960.14499101357780428No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG930.1404600444034979No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG920.1389497213453958No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG890.1344187521710894No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG870.13139810605488514No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG860.129887782996783No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG860.129887782996783No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG850.12837745993868088No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG820.1238464907643745No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG820.1238464907643745No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG800.12082584464817023No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG800.12082584464817023No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG800.12082584464817023No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG790.11931552159006813No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG780.11780519853196599No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG780.11780519853196599No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG760.11478455241576173No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG760.11478455241576173No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG750.11327422935765959No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG750.11327422935765959No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG750.11327422935765959No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG740.11176390629955749No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG740.11176390629955749No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG740.11176390629955749No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG730.11025358324145534No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG730.11025358324145534No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG730.11025358324145534No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG730.11025358324145534No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG720.10874326018335322No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC720.10874326018335322No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG720.10874326018335322No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG710.10723293712525109No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG710.10723293712525109No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG700.10572261406714897No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG700.10572261406714897No Hit
GANTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG690.10421229100904685No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA680.1027019679509447No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG670.10119164489284257No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)