FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008119986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67729
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5780.8534010542013023No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5240.7736715439472014No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4400.649647861329711No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4320.6378360820328073No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4150.6127360510268866No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4000.5905889648451919No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3960.58468307519674No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3800.5610595166029323No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3770.5566300993665934No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3530.5211947614758818No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3400.5020006201184131No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3330.4916653132336223No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3310.4887123684093963No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3280.48428295117305736No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3130.4621358649913626No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2950.43555936157332903No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2820.41636522021586025No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2750.4060299133310694No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2650.3912651892099396No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2500.36911810302824494No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2470.364688685791906No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2470.364688685791906No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2440.36025926855556706No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2190.3233474582527426No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2150.3174415686042906No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2080.3071062617194998No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2030.29972389965893487No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2010.29677095483470894No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1800.26576503418033637No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1440.2126120273442691No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1430.2111355549321561No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1430.2111355549321561No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1420.20965908252004312No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1250.18455905151412247No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1250.18455905151412247No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1130.1668413825687667No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1060.15650607568397584No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1050.15502960327186285No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1040.1535531308597499No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1020.15060018603552391No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1010.14912371362341095No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG990.146170768799185No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG970.14321782397495902No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG960.14174135156284604No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG890.1314060446780552No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG890.1314060446780552No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC880.1299295722659422No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG850.12550015502960327No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG840.12402368261749028No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG830.12254721020537732No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG800.11811779296903838No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC780.11516484814481241No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC770.11368837573269942No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG770.11368837573269942No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG770.11368837573269942No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG770.11368837573269942No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG760.11221190332058645No Hit
NNNTNNTCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC750.11073543090847346No Hit
GNNTANTCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC750.11073543090847346No Hit
GNNTANTGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC700.10335306884790857No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG690.1018765964357956No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC690.1018765964357956No Hit
NNNTNNTGGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC680.10040012402368262No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG680.10040012402368262No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG680.10040012402368262No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)