FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008119988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27995
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4181.493123772102161No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3281.1716377924629398No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3281.1716377924629398No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3131.118056795856403No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3011.0751919985711735No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2640.9430255402750491No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2490.8894445436685123No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2350.8394356135024112No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2020.72155742096803No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1360.4858010358992677No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1260.45008037149490987No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1190.42507590641185933No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1000.3572066440435792No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG960.3429183782818361No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG910.3250580460796571No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG900.3214859796392213No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG850.30362564743704235No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG840.30005358099660656No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG810.28933738167529915No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG760.27147704947312024No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG740.2643329165922486No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG720.25718878371137704No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG650.2321843186283265No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG650.2321843186283265No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG590.21075191998571174No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA590.21075191998571174No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG580.20717985354527596No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG580.20717985354527596No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG560.20003572066440434No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG560.20003572066440434No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG560.20003572066440434No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG550.19646365422396855No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG540.19289158778353277No Hit
NNNTNNCGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG540.19289158778353277No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG540.19289158778353277No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG530.18931952134309699No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG510.1821753884622254No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG510.1821753884622254No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG500.1786033220217896No Hit
GANTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG500.1786033220217896No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG490.17503125558135382No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG480.17145918914091804No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG480.17145918914091804No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG480.17145918914091804No Hit
NNCTNNTAGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG480.17145918914091804No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG480.17145918914091804No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG470.16788712270048223No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG470.16788712270048223No Hit
GNNTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG470.16788712270048223No Hit
GNNTANTAGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG470.16788712270048223No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG470.16788712270048223No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG460.16431505626004644No Hit
NANTNNCAGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG450.16074298981961066No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG450.16074298981961066No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG450.16074298981961066No Hit
NANTANTAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG440.15717092337917485No Hit
GANTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG440.15717092337917485No Hit
NANTNNACGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG440.15717092337917485No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG440.15717092337917485No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG430.15359885693873906No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG430.15359885693873906No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG420.15002679049830328No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG420.15002679049830328No Hit
NNNTNNCAGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG420.15002679049830328No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG410.14645472405786747No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG400.14288265761743169No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG400.14288265761743169No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG400.14288265761743169No Hit
GANTNNTAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG390.1393105911769959No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG390.1393105911769959No Hit
GANTANCAGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG390.1393105911769959No Hit
NNCTNNAGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG380.13573852473656012No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG380.13573852473656012No Hit
NNCTNNCGGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG380.13573852473656012No Hit
NANTANCGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG370.1321664582961243No Hit
NNCTNNCAGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG370.1321664582961243No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG370.1321664582961243No Hit
GNNTANCGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG360.12859439185568852No Hit
NNNTNNACGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG360.12859439185568852No Hit
NNNTNNCAGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG360.12859439185568852No Hit
GANTANCGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG360.12859439185568852No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA350.12502232541525274No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG350.12502232541525274No Hit
GANTNNAGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG350.12502232541525274No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG340.12145025897481693No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG340.12145025897481693No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG340.12145025897481693No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG340.12145025897481693No Hit
NNNTNNACGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG340.12145025897481693No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG340.12145025897481693No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG330.11787819253438114No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG330.11787819253438114No Hit
GANTANAGGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG330.11787819253438114No Hit
NANTNNAGGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG330.11787819253438114No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG330.11787819253438114No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA320.11430612609394536No Hit
GANTNNCAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG320.11430612609394536No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG320.11430612609394536No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG320.11430612609394536No Hit
GANTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG320.11430612609394536No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG310.11073405965350955No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG310.11073405965350955No Hit
NNNTNNCCGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG310.11073405965350955No Hit
GANTANCCGGGTNTCTNNTNNTGTTCGCTCCCCATGNTTTCGCTTCTCAG310.11073405965350955No Hit
NANTANACGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG310.11073405965350955No Hit
GNNTANCCGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG310.11073405965350955No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA310.11073405965350955No Hit
NNNTNNAGGGGTATNTNNTNNTGTTCGNTCNCCNTGNTTTCGCTTCTCAG310.11073405965350955No Hit
NNCTNNCCGGGTATNTANTCNTGTTCGNTCNCCNTGCTTTCGCTTCTCAG300.10716199321307376No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG300.10716199321307376No Hit
NANTANCCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG300.10716199321307376No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG300.10716199321307376No Hit
NNNTNNCCGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG300.10716199321307376No Hit
GANTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG300.10716199321307376No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG300.10716199321307376No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG290.10358992677263798No Hit
NANTANAGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG290.10358992677263798No Hit
GANTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG290.10358992677263798No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG290.10358992677263798No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG290.10358992677263798No Hit
NNNTNNAGGGNNNTNTNATNNTGTTCGNTCNCCNTGNTTTCGCTTCNNNG290.10358992677263798No Hit
GANTNNACGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTTCNCAG290.10358992677263798No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG290.10358992677263798No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG290.10358992677263798No Hit
GNNTANAGGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG290.10358992677263798No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG280.10001786033220217No Hit
NANTANCAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTTCTCAG280.10001786033220217No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG280.10001786033220217No Hit
GNNTANCAGGGTNTNTNNTNNTGTTCGNTCNCNNTGNTTTCGCTTCTNNG280.10001786033220217No Hit
NANTNNAAGGGTNTNTNNTCNTGTNCGNTCNCCNTGCNTTCGCTTCTCAG280.10001786033220217No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG280.10001786033220217No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)