FastQCFastQC Report
Wed 8 May 2024
EGAF00008119992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43767
Total Bases13.1 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3160.7220051637078163No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2750.6283272785431946No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2350.5369342198460028No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2340.5346493933785729No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2260.5163707816391345No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1940.443256334681381No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1810.4135535906047936No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1590.363287408321338No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1330.30388192016816323No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1310.2993122672333036No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1230.2810336554938652No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1170.26732469668928643No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1140.26047021728699704No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1140.26047021728699704No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1110.25361573788470765No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1050.23990677908012886No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1040.23762195261269906No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1030.23533712614526928No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1010.23076747321040966No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG950.21705851440583088No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG930.2124888614709713No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG920.21020403500354146No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG910.20791920853611168No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG870.19877990266639248No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG850.1942102497315329No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG830.18964059679667328No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG820.1873557703292435No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG810.1850709438618137No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG790.18050129092695408No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG790.18050129092695408No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG760.1736468115246647No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG760.1736468115246647No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG750.1713619850572349No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC750.1713619850572349No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG740.1690771585898051No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG740.1690771585898051No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG740.1690771585898051No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG740.1690771585898051No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG720.16450750565494549No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG710.1622226791875157No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG690.15765302625265612No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG690.15765302625265612No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG670.1530833733177965No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG670.1530833733177965No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG660.15079854685036673No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG650.1485137203829369No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG650.1485137203829369No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG640.1462288939155071No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG640.1462288939155071No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG640.1462288939155071No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG630.14394406744807733No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG630.14394406744807733No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG630.14394406744807733No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG620.14165924098064753No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG610.13937441451321772No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG610.13937441451321772No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC600.1370895880457879No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG600.1370895880457879No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG590.13480476157835813No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG590.13480476157835813No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG580.13251993511092833No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC580.13251993511092833No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG580.13251993511092833No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG570.13023510864349852No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG550.12566545570863893No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG550.12566545570863893No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG550.12566545570863893No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG550.12566545570863893No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG540.12338062924120913No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG540.12338062924120913No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG540.12338062924120913No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG530.12109580277377932No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG520.11881097630634953No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC520.11881097630634953No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG520.11881097630634953No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG510.11652614983891975No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG510.11652614983891975No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG500.11424132337148993No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG500.11424132337148993No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG500.11424132337148993No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC500.11424132337148993No Hit
GANTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG500.11424132337148993No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG490.11195649690406015No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG490.11195649690406015No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG490.11195649690406015No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG490.11195649690406015No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG490.11195649690406015No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG480.10967167043663033No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG480.10967167043663033No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG470.10738684396920053No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG470.10738684396920053No Hit
NANTANAGGGGTATNTANTNNTGTTTGNTANCCNNGNTTTCGCGCTTCAG460.10510201750177073No Hit
GANTNNACGGGTATNTAATNNTGTTTGNTANCCNNGCTTTCGCGCTNCAG450.10281719103434094No Hit
NNNTNNACGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG450.10281719103434094No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG450.10281719103434094No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG450.10281719103434094No Hit
GNNTANAGGGGTNTNTNNTNNTGTTTGNTANCNNCGNTTTCGCGCTTNNG450.10281719103434094No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG440.10053236456691116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)