FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008119994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008119994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60573
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG5310.8766282006834729No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4600.7594142604790913No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3850.6355967180096743No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3750.6190877123470854No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3100.5117791755402572No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2820.4655539596850082No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2800.46225215855249036No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2690.4440922523236425No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2430.4011688376009113No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2360.389612533637099No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2340.38631073250458126No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2280.3764053291070279No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2200.3631981245769567No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2100.34668911891436777No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1900.3136711075891899No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1860.30706750532415433No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1660.27404949399897643No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1650.27239859343271755No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1630.2690967923001998No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1600.2641440906014231No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1560.2575404883363875No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1520.2509368860713519No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1440.23772968154128077No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1400.23112607927624518No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1390.2294751787099863No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1270.20966437191487958No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1250.20636257078236178No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1240.20471167021610287No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1230.203060769649844No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1230.203060769649844No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1190.19645716738480842No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1090.17994816172221947No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1060.1749954600234428No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1030.17004275832466612No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1020.1683918577584072No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC930.15353375266207717No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG900.1485810509633005No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG890.14693015039704158No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG860.1419774486982649No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG860.1419774486982649No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG750.12381754246941706No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG750.12381754246941706No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG700.11556303963812259No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG690.1139121390718637No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC670.11061033793934591No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG670.11061033793934591No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG670.11061033793934591No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG670.11061033793934591No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTANCCNCGNTTTCGCGCTNNNG660.10895943737308703No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG660.10895943737308703No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG660.10895943737308703No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG650.10730853680682813No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTANCCNCGCTTTCGCGCTTCAG650.10730853680682813No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG640.10565763624056923No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG640.10565763624056923No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG630.10400673567431033No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG620.10235583510805144No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG620.10235583510805144No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)