FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116674
Sequences flagged as poor quality0
Sequence length301
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG78546.7315768723108835No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG72276.194182079983544No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG57454.923976207209833No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG56714.860551622469444No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG48364.144882321682637No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG45413.8920410717040643No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG43463.724908720023313No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG37463.2106553302363854No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG32362.7735399489174966No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG16371.4030546651353344No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG15581.3353446354800556No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12471.0687899617738315No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG12031.03107804652279No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG10880.9325128134802956No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG9550.8185199787441931No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9140.7833793304420865No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8770.7516670380718926No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8560.7336681694293501No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8250.7070984109570255No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6800.5828205084251847No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6300.5399660592762741No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6170.5288239024975573No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5940.5091108558890585No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5530.47397020758695163No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4280.36683408471467505No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4270.36597699573169684No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4200.35997737285084935No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4100.35140648302106725No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4040.34626394912319797No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3070.26312631777431134No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2840.24341327116581243No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2300.19713046608498894No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2150.18427413134031576No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2150.18427413134031576No Hit
GACTACTCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCCTCAG2130.18255995337435932No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2020.17313197456159898No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGTGCCTCAG1920.16456108473181685No Hit
GACTACTGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCCTCAG1900.16284690676586044No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1870.16027563981692577No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGTGCCTCAG1870.16027563981692577No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1860.1594185508339476No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGTGCCTCAG1850.15856146185096937No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1810.15513310591905652No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1750.14999057202118724No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1690.14484803812331795No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG1650.1414196821914051No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1640.1405625932084269No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1610.13799132625949226No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG1500.12856334744673192No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1490.1277062584637537No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1490.1277062584637537No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGTGCCTCAG1470.12599208049779728No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1450.12427790253184084No Hit
GACTACCGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCCTCAG1390.11913536863397158No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1380.11827827965099336No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1310.11227865677014587No Hit
GACTACTAGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCCTCAG1300.11142156778716765No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGTGCCTCAG1280.10970738982121124No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGNTTTCGTGCCTCAG1280.10970738982121124No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1250.1071361228722766No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1230.10542194490632016No Hit
GACTACCCGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCCTCAG1230.10542194490632016No Hit
GACTACAGGGGTATCTAATCNTGTTTGCTCCCCACGCTTTCGTGCCTCAG1230.10542194490632016No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1210.10370776694036375No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGTGCCTCAG1210.10370776694036375No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1180.10113649999142912No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1170.1002794110084509No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)