FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85882
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17982.0935702475489624No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG17312.015556228313267No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13711.596376423464754No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12581.4648005402761928No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG12401.4438415500337671No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG12151.4147318413637315No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG11251.3099368901516033No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG11121.2947998416431847No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9451.1003469877273468No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9051.0537714538552898No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8711.0141822500640414No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8540.9943876481684171No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8540.9943876481684171No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8020.933839454134743No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7960.9268531240539345No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG7790.9070585221583102No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7780.9058941338115089No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7490.8721268717542675No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6840.7964416292121749No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6750.785962134090962No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6640.7731538622761464No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6550.7626743671549335No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6340.7382222118721036No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6180.7195919983232808No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6070.7067837265084651No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6010.6997973964276566No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5790.6741808527980252No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5750.6695232994108195No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5600.6520574742087981No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5580.6497286975151952No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG5450.6345916490067768No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5260.6124682704175497No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4780.5565776297710813No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4620.5379474162222585No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4560.5309610861414499No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4300.5006869891246128No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4140.48205677557579No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4030.4692485037609744No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3960.4610977853333644No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3840.4471251251717473No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3740.43548124170373304No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3690.4296592999697259No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3510.40870030972730026No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3170.3691111059360518No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2640.30739852355557623No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1950.2270557276262779No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1660.1932884655690366No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG1510.1758226403670152No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1420.16534314524580238No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1390.1618499802053981No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1300.15137048508418527No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1240.14438415500337673No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG1240.14438415500337673No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1210.14089098996297245No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1150.1339046598821639No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG1140.13274027153536247No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1090.12691832980135534No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1070.1245895531077525No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1060.12342516476095107No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGAGCCTCAG1050.12226077641414965No Hit
GACTACCAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1040.12109638806734822No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG1040.12109638806734822No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1010.11760322302694394No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG1000.11643883468014252No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG990.1152744463333411No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG990.1152744463333411No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG980.11411005798653967No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG960.11178128129293682No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCNCCACGCTTTCGAGCCTCAG940.10945250459933396No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCCTCAG940.10945250459933396No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG940.10945250459933396No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG940.10945250459933396No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCNCCACGCTTTCGAGCCTCAG910.1059593395589297No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCNCCACGCTTTCGCACATCAG900.10479495121212827No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG890.10363056286532685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)