FastQCFastQC Report
Wed 2 Aug 2023
EGAF00008120020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66217
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG17212.5990304604557743No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG15102.280381170998384No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG13432.0281800746031986No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG12051.8197743781808298No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10551.5932464472869505No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG10401.5705936541975625No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG9531.4392074542791127No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG8551.2912092060951115No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG6330.9559478683721703No Hit
GNNTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4380.6614615582101273No Hit
GNNTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4190.6327680202969026No Hit
GNNTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3420.5164836824380445No Hit
GNNTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3050.46060679281755446No Hit
GNNTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2770.41832157905069695No Hit
GNNTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2710.40926046181494175No Hit
GANTANTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2590.3911382273434315No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2550.3850974825195947No Hit
GNNTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2530.38207711010767625No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG2450.3699956204600027No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG2330.3518733859884924No Hit
GANTANTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2190.33073077910506365No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2130.3216696618693085No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG2100.3171391032514309No Hit
GNNTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2090.31562891704547175No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG2060.31109835842759415No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG2050.3095881722216349No Hit
GANTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2040.30807798601567576No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2010.30354742739779816No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG2000.30203724119183895No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG2000.30203724119183895No Hit
GNNTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1970.29750668257396135No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1960.2959964963680022No Hit
GANTANCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1960.2959964963680022No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1960.2959964963680022No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1900.286935379132247No Hit
NNNTNNTCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1890.2854251929262878No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1870.28240482051436944No Hit
GANTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1860.2808946343084102No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1850.27938444810245106No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1830.2763640756905327No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1800.2718335170726551No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1800.2718335170726551No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1790.27032333086669585No Hit
NNCTNNCGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1780.2688131446607367No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1760.26579277224881825No Hit
GANTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1750.2642825860428591No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1740.2627723998368999No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1680.2537112826011447No Hit
GANTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1670.2522010963951855No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1620.24465016536538955No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1610.24313997915943034No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1610.24313997915943034No Hit
GNNTANCGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1610.24313997915943034No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1590.24011960674751195No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1570.23709923433559357No Hit
GANTNNCGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1560.23558904812963438No Hit
GANTANCAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1550.23407886192367522No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1540.232568675717716No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1520.22954830330579762No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1510.2280381170998384No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1510.2280381170998384No Hit
GANTANAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1480.2235075584819608No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1480.2235075584819608No Hit
GANTANTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1480.2235075584819608No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1480.2235075584819608No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1440.21746681365812404No Hit
NNNTNNTAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1420.21444644124620568No Hit
GANTANTAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1420.21444644124620568No Hit
GANTANCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1410.21293625504024646No Hit
GANTANCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1410.21293625504024646No Hit
GANTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1400.21142606883428725No Hit
GANTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1400.21142606883428725No Hit
NANTNNAGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1390.20991588262832808No Hit
GNNTANTAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1370.2068955102164097No Hit
NNNTNNCCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1340.2023649515985321No Hit
GANTANCCGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1340.2023649515985321No Hit
NANTNNCAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1340.2023649515985321No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1300.1963242067746953No Hit
NANTANTAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1300.1963242067746953No Hit
NNCTNNCAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1290.19481402056873615No Hit
NANTANCCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1280.19330383436277693No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1260.19028346195085855No Hit
NNNTNNCAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1240.18726308953894014No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1230.18575290333298095No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1230.18575290333298095No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1200.18122234471510337No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1200.18122234471510337No Hit
GANTANAGGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG1180.178201972303185No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1150.1736714136853074No Hit
NNCTNNAGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG1150.1736714136853074No Hit
GANTANACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1150.1736714136853074No Hit
NNNTNNCCGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1140.1721612274793482No Hit
GANTNNAGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1140.1721612274793482No Hit
NNNTNNAGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG1130.170651041273389No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1130.170651041273389No Hit
GNNTANAGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1130.170651041273389No Hit
GANTNNCAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1120.16914085506742982No Hit
NANTNNACGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG1100.1661204826555114No Hit
NANTANAGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG1100.1661204826555114No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1090.16461029644955222No Hit
GANTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1090.16461029644955222No Hit
NNNTNNCAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1080.16310011024359303No Hit
GANTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1060.16007973783167465No Hit
NNNTNNAGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1040.15705936541975626No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1020.15403899300783788No Hit
GNNTANCAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG1010.15252880680187866No Hit
NNNTNNACGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG1000.15101862059591947No Hit
GANTNNAAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG1000.15101862059591947No Hit
GNNTANACGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG990.14950843438996028No Hit
NNNTNNAAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG980.1479982481840011No Hit
NANTANCAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG970.14648806197804187No Hit
GANTANAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG960.14497787577208268No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG930.1404473171542051No Hit
GNNTANAAGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGCACATNNG920.13893713094824592No Hit
GANTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG920.13893713094824592No Hit
GANTANACGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG910.13742694474228673No Hit
NNNTNNACGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGCACATCAG880.13289638612440913No Hit
GANTANAAGGGTNTCTNNTNNTGTTTGATCCCCACGNTTTCGCACATCAG880.13289638612440913No Hit
NNNTNNAAGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGCACANNNG870.13138619991844994No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG860.12987601371249075No Hit
GANTNNACGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGCACANCAG860.12987601371249075No Hit
NNCTNNACGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG820.12383526888865397No Hit
NANTANACGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG800.12081489647673559No Hit
NANTANAAGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGCACATCAG750.11326396544693962No Hit
NANTNNAAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGCACATCAG740.1117537792409804No Hit
NNCTNNAAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGCACATCAG710.10722322062310284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)