FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139165
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG89046.3981604570114605No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG81865.882226134444724No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG68114.894190349585025No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG63724.57873746990982No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG56164.035497431106959No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG50793.649624546401754No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG49383.5483059677361406No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG43013.090575935041138No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG37172.670930190780728No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13140.9442029245859231No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12700.9125857794704129No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC10000.7185714798979628No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9130.65605576114684No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8990.6459957604282686No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8100.5820428987173499No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6500.4670714619336758No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6440.46276003305428803No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6430.4620414615743901No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG5810.4174900298207164No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG5550.3988071713433694No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG4570.328387166313369No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG4420.3176085941148996No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG4170.2996443071174505No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG3820.2744943053210218No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3480.2500628750044911No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3450.24790716056479717No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG3400.2443143031653074No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG3360.2414400172457155No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG3260.2342543024467359No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG3060.21988287284877667No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2750.19760715697193978No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2730.19617001401214385No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2690.19329572809255202No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG2680.19257715661265404No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTTCTCAG2570.18467287033377644No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2470.17748715553479683No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2290.1645528688966335No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG2280.16383429741673555No Hit
GACTACTGGGGTATCTAATCNTGTTCGNTCCCCACGNTTTCGCTCCTCAG2270.16311572593683757No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG2260.16239715445693959No Hit
GACTACTCGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG2230.16024144001724572No Hit
GACTACTGGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG2170.15593001113785793No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2160.15521143965795997No Hit
GACTACTCGGGTATCTAATCNTGTTCGNTCCCCACGNTTTCGCTCCTCAG2120.15233715373836812No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG2040.14658858189918442No Hit
GACTACCGGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1990.1429957244996946No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1990.1429957244996946No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1970.1415585815398987No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1890.135810009700715No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1880.135091438220817No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1840.13221715230122516No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1780.1279057234218374No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1740.12503143750224555No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCAG1680.12072000862285776No Hit
GACTACTAGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1670.12000143714295979No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1670.12000143714295979No Hit
GACTACTAGGGTATCTAATCNTGTTCGNTCCCCACGNTTTCGCTCCTCAG1610.11569000826357201No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1590.11425286530377608No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1590.11425286530377608No Hit
GACTACCAGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1560.1120971508640822No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1560.1120971508640822No Hit
GACTACCGGGGTATCTAATCNTGTTCGNTCCCCACGNTTTCGCTCCTCAG1560.1120971508640822No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1540.11066000790428629No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCTCCTCAG1510.10850429346459238No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCATGAG1500.10778572198469442No Hit
GACTACCCGGGTATCTAATCNTGTTCGNTCCCCACGNTTTCGCTCCTCAG1480.1063485790248985No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAG1450.10419286458520462No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1420.10203715014551072No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1420.10203715014551072No Hit
GACTACCCGGGTATCTAATCNTGTTCGCTCCCCACGCTTTCGCTCCTCAG1410.10131857866561277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)