FastQCFastQC Report
Wed 8 May 2024
EGAF00008120068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175408
Total Bases52.7 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG60363.4411201313509077No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG55373.156640518106358No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG45902.616756362309587No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG45142.5734288059837636No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG36182.0626197208793213No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG34811.9845160996077718No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG34761.9816656024810728No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG34371.9594317248928212No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG34071.942328742132628No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG30701.7502052357931224No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG29971.7085879777433186No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG26531.5124737754264344No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG26501.510763477150415No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG25551.456604031743136No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG25291.4417814466843017No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG24121.3750798139195477No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG22861.3032472863267353No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG20591.1738347167746055No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG20291.156731734014412No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG19991.1396287512542187No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG19391.105422785733832No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18761.0695065219374258No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG18381.0478427437745141No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG16050.9150095776703457No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG15450.880803612149959No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG14920.8505883426069507No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG14380.8198029736386026No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13660.7787558150141385No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG12920.7365684575389948No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG12580.7171850770774423No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG10430.5946137006293898No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG8930.5090987868284229No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8650.493136002918909No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG8640.4925659034935693No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8580.48914530694153063No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG8130.46349083280124054No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7920.45151874486910515No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG7630.43498586153425156No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7470.42586427072881505No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7410.4224436741767764No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6530.37227492474687585No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6510.3711347258961963No Hit
GANTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6440.36714402991881784No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6330.36087293624008027No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6180.35232144485998357No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG6150.35061114658396425No Hit
GANTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6130.3494709477332847No Hit
GANTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5790.3300875672717322No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5720.3260968712943538No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5070.28904040864726804No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG5040.2873301103712487No Hit
GANTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4900.27934871841649184No Hit
GANTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4740.2702271276110554No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4660.26566633220833713No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4580.2611055368056189No Hit
GANTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4410.2514138465748426No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4370.24913344887348352No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA4320.24628295174678466No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4270.24343245462008575No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4200.23944175864270728No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4170.23773146036668796No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4140.2360211620906686No Hit
GANTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4130.23545106266532884No Hit
GANTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG4050.2308902672626106No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3970.22632947185989238No Hit
GANTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3830.21834807990513544No Hit
GANTANAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3760.214357383927757No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3680.20979658852503877No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3430.1955441028915443No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG3430.1955441028915443No Hit
GANTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3390.19326370519018515No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3360.19155340691416584No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3240.18471221381008848No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA3100.17673082185533157No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3060.17445042415397244No Hit
GACTANTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2990.170459728176594No Hit
GACTACTGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2970.16931952932591443No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2870.16361853507251664No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2850.16247833622183708No Hit
GACTANAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2800.1596278390951382No Hit
GACTACTCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2750.1567773419684393No Hit
GACTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2750.1567773419684393No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2700.1539268448417404No Hit
GACTANCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2620.14936604943902215No Hit
GACTANCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2590.14765575116300284No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2580.14708565173766305No Hit
GACTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2550.1453753534616437No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2520.14366505518562436No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2480.14138465748426526No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2470.14081455805892548No Hit
GACTACCGGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2460.1402444586335857No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2400.136823862081547No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2370.1351135638055277No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2310.131692967253489No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2290.13055276840280944No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2240.12770227127611056No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACATGAG2230.12713217185077078No Hit
GACTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2190.12485177414941165No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2150.12257137644805254No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2140.12200127702271277No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2120.1208610781720332No Hit
GANTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2110.12029097874669342No Hit
GACTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2060.11744048161999453No Hit
GACTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2060.11744048161999453No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2020.11516008391863541No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2010.11458998449329562No Hit
GACTACCCGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG2000.11401988506795585No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG2000.11401988506795585No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1960.11173948736659674No Hit
GACTANAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1930.1100291890905774No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1910.10888899023989784No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1900.10831889081455806No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1890.10774879138921829No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1850.10546839368785917No Hit
GACTANACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1840.10489829426251938No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1830.10432819483717962No Hit
GACTACTAGGGTATCTAATCCCGTTCGCTCCCCTGGCTTTCGCGCCTCAG1810.10318799598650005No Hit
GACTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1800.10261789656116026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)