FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008120074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110786
Sequences flagged as poor quality0
Sequence length301
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG23682.137454190962757No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG21401.9316520137923565No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC20601.8594407235571282No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC19041.7186287075984332No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG17791.605798566605889No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG17091.542613687650064No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC15951.4397125990648638No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG14841.3395194338634846No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC13781.243839474301807No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG13491.217662881591537No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13091.1815572364739226No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG13021.1752387485783402No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG12281.108443305110754No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC12221.1030274583431119No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC11971.0804614301446032No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG11121.0037369342696731No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10420.9405520553138483No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG10140.9152781037315184No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCATGCTTTCGCTCCTCAG9970.8999332045565325No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9870.8909067932771289No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9440.8520932247756937No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG9050.8168902207860198No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8800.7943241925875111No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG8010.7230155434802231No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7950.7175996967125811No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG7420.6697597169317423No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG7030.6345567129420685No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6530.5894246565450508No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG6480.584911450905349No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG6040.5451952412759735No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG5890.5316556243568682No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5330.48110772119220835No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG5260.474789233296626No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4940.4459047172025346No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4730.42694925351578716No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4710.4251439712599065No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4650.4197281244922644No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG4500.40618850757315905No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4480.4043832253172784No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4290.3872330438864116No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG4120.37188814471142567No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG4040.3646670156879028No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3470.3132164713953027No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3450.31141118913942195No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3260.29426100770855523No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3150.28433195530121136No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG3150.28433195530121136No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG3110.28072139078944996No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2980.2689870561262253No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2910.26266856823064283No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2760.24912895131153756No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2760.24912895131153756No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2670.24100518116007438No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2470.2229523586012673No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2400.21663387070568482No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2390.2157312295777445No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2360.21302330619392343No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA2320.209412741682162No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG2310.20851010055422167No Hit
GACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2290.20670481829834095No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG2270.20489953604246025No Hit
GACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG2210.19948368927481813No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2200.19858104814687777No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2080.18774935461159353No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1990.17962558446013036No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1950.17601501994836893No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1910.1724044554366075No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1900.17150181430866718No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1810.16337804415720397No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1790.16157276190132328No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1700.1534489917498601No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1690.15254635062191974No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1680.1516437094939794No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1670.15074106836603904No Hit
GACTACTCGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1660.14983842723809868No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1640.14803314498221795No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1550.13990937483075477No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1500.13539616919105302No Hit
GACTACTGGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1490.13449352806311266No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAA1480.13359088693517232No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1470.13268824580723196No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1470.13268824580723196No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1450.13088296355135126No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1370.12366183452782843No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1350.12185655227194771No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1320.11914862888812666No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1290.11644070550430559No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1290.11644070550430559No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1260.11373278212048453No Hit
GACTACTAGGGTATCTAATCCTAAGACCTTCTTCCTCCACGCGGCGTCGC1240.11192749986460382No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1240.11192749986460382No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1240.11192749986460382No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAG1230.11102485873666348No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1230.11102485873666348No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1230.11102485873666348No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1220.11012221760872312No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1220.11012221760872312No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1190.10741429422490208No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCGCCTCAC1180.10651165309696171No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAGCATGAA1180.10651165309696171No Hit
GACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCAG1170.10560901196902137No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1150.10380372971314064No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)