FastQCFastQC Report
Wed 8 May 2024
EGAF00008120090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90928
Total Bases27.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG55706.125725849023403No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG52785.804592644729896No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG41824.599243357381664No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG40924.500263945099419No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG36814.048257962343833No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG34593.8041087453809608No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG31213.432386063698751No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG28203.101354918177019No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG26662.9319901460496216No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC9671.0634787964103467No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC8730.9601002991377793No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG7570.832526834418441No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC7300.8028330107337673No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG6690.7357469646313567No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC6110.6719602322716874No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5920.6510645785676579No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5860.6444659510821749No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC5800.637867323596692No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG5130.5641826500087982No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4770.5245908850959001No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4600.5058947738870315No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4550.500395917649129No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4420.4860988914305825No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4220.4641034664789724No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG4200.4619039239838114No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3570.39261833538623964No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3010.33103114552173146No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2840.3123350343128629No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2760.3035368643322189No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2680.2947386943515749No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2660.2925391518564138No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG2300.25294738694351576No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2240.24634875945803272No Hit
GACTACCAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA2180.2397501319725497No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1980.21775470702093966No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1950.21445539327819815No Hit
GACTACTGGGGTATCTAATCNTGTTCGCTCCCCATGCTTTCGCTTCTCAG1870.20565722329755412No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1820.20015836705965157No Hit
GACTACCGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1810.19905859581207108No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1750.19245996832658807No Hit
GACTACTCGGGTATCTAATCNTGTTCGCTCCCCATGCTTTCGCTTCTCAG1740.19136019707900756No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGNTTTCGCTTCTCAG1660.18256202709836356No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1580.1737638571177195No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGNTTTCGCTTCTCAG1530.168265000879817No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1500.1649656871370755No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1470.16166637339433398No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1390.15286820341368995No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1310.14407003343304592No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1280.1407707196903044No Hit
GACTACTCGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA1280.1407707196903044No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTCCTCAG1270.1396709484427239No Hit
GACTACCGGGGTATCTAATCNTGTTCGCTCCCCATGCTTTCGCTTCTCAG1260.13857117719514342No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1260.13857117719514342No Hit
GACTACAGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1260.13857117719514342No Hit
GACTACACGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA1250.1374714059475629No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGNTTTCGCTTCTCAG1220.1341720922048214No Hit
GACTACTAGGGTATCTAATCNTGTTCGCTCCCCATGCTTTCGCTTCTCAG1180.1297730072144994No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1160.12757346471933836No Hit
GACTACCAGGGTATCTAATCNTGTTCGCTCCCCATGCTTTCGCTTCTCAG1150.12647369347175788No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1140.12537392222417737No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGNTTTCGCTTCTCAG1130.12427415097659687No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1120.12317437972901636No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGNTTTCGCTTCTCAG1100.12097483723385535No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG1090.11987506598627486No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGNTTTCGCTTCTCAG1080.11877529473869435No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG980.10777758226288932No Hit
GACTACCCGGGTATCTAATCNTGTTCGCTCCCCATGCTTTCGCTTCTCAG980.10777758226288932No Hit
GACTACTAGGGTATCTAATCCCAAGTCTACACATCTGACGCTGCCGACGA960.10557803976772831No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG940.1033784972725673No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGNTTTCGCTTCTCAG940.1033784972725673No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG930.10227872602498679No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGNTTTCGCTTCTCAG920.1011789547774063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)