FastQCFastQC Report
Wed 8 May 2024
EGAF00008120110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54464
Total Bases16.3 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3850.7068889541715629No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3030.5563307873090482No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2790.512264982373678No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2700.49574030552291426No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2450.44983842538190366No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2330.42780552291421853No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2160.39659224441833135No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2090.3837397179788484No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1840.33783783783783783No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1770.32498531139835485No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1660.3047884841363102No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1650.30295240893066977No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1550.2845916568742656No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1430.2625587544065805No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1420.26072267920094006No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1170.21482079905992948No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1140.20931257344300822No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1010.18544359576968272No Hit
GNNTANTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1000.1836075205640423No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1000.1836075205640423No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG980.17993537015276145No Hit
GACTACTCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA920.16891891891891891No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC880.16157461809635723No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG820.1505581668625147No Hit
GNNTANCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC810.14872209165687425No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG810.14872209165687425No Hit
NNNTNNTCGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC790.14504994124559342No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG770.14137779083431257No Hit
GNNTANTCGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC770.14137779083431257No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC750.1377056404230317No Hit
GACTACTGGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA750.1377056404230317No Hit
GACTACTAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA750.1377056404230317No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC730.13403349001175088No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG690.1266891891891892No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG660.12118096357226793No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC660.12118096357226793No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG640.11750881316098707No Hit
GNNTANTGGGGTNTNTNNTNNGAAGACNTTNTNNCTNCACGCGGCGTNNC630.11567273795534666No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG630.11567273795534666No Hit
GACTACCCGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA620.11383666274970623No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC610.1120005875440658No Hit
NNNTNNTCGGGTATNTNNTNNGAAGACNTTNTTNCTNCACGCGGCGTCGC600.11016451233842538No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG600.11016451233842538No Hit
NNCTNNTGGGGTATNTANTCNGAAGACNTTNTTNCTCCACGCGGCGTCGC600.11016451233842538No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC600.11016451233842538No Hit
GACTACAAGGGTATCTAATCCGCTATGTACACATCTGACGCTGCCGACGA590.10832843713278496No Hit
GANTNNTCGGGTATNTAATNNGAAGACNTTNTTNNTCCACGCGGCGNCGC580.10649236192714452No Hit
NNNTNNTAGGNNNTNTNATNNGAAGACNTTNTTNCTNCACGCGGCGNNNC570.10465628672150411No Hit
GANTANTGGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC570.10465628672150411No Hit
GANTANTAGGGTNTCTNNTNNGAAGACCTTCTTCCTNCACGCGGCGTCGC570.10465628672150411No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG560.1028202115158637No Hit
GANTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC550.10098413631022325No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)