FastQCFastQC Report
Wed 8 May 2024
EGAF00008120112

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120112
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61858
Total Bases18.6 Mbp
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4600.7436386562772802No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG4480.7242393869830903No Hit
GACTACTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC4200.6789744252966472No Hit
GACTACTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC3850.6223932231885932No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3430.5544957806589286No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3200.5173138478450645No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG3040.4914481554528113No Hit
GACTACCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2940.47528209770765306No Hit
GACTACTAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2900.4688156746095897No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2780.4494164053153998No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2740.4429499822173365No Hit
GACTACCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2710.438100164893789No Hit
GACTACCAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2610.4219341071486307No Hit
GACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2280.36858611658960844No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG2070.3346373953247761No Hit
GACTACTCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG2050.33140418377574443No Hit
GACTACAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC2040.3297875780012286No Hit
GACTACACGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1880.3039218856089754No Hit
GACTACTGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1720.2780561932167222No Hit
GACTACAAGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1660.2683565585696272No Hit
GACTACTAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1420.22955801998124736No Hit
GACTACCGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1330.21500856801060494No Hit
GNNTANTGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC1240.2004591160399625No Hit
GACTACCCGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1110.17944324097125675No Hit
GACTACAGGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1090.17621002942222508No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1080.17459342364770927No Hit
GACTACCAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG1010.1632771832260985No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG1000.16166057745158266No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG960.15519415435351935No Hit
GNNTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC890.14387791393190857No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG850.13741149083384524No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG850.13741149083384524No Hit
GACTACACGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG840.13579488505932943No Hit
GNNTANCCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC810.13094506773578196No Hit
GNNTANCGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC800.12932846196126613No Hit
GACTACAAGGGTATCTAATCCTGTTTGATACCCACGCTTTCGTGCTTCAG790.12771185618675032No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG780.12609525041223446No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG770.12447864463771864No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG730.11801222153965535No Hit
GANTANTCGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC720.1163956157651395No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG710.11477900999062368No Hit
NANTNNTCGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC690.11154579844159203No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG690.11154579844159203No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCAG690.11154579844159203No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTACCCACGNTTTCGCGCTTCAG670.10831258689256039No Hit
NANTNNTGGGGTNTNTNNTCNGAANACNTTNTTNCTCNACGCGGCGTCGC660.10669598111804454No Hit
GNNTANAGGGGTATCTAATCCGAAGACCTTCTTCCTCCACGCGGCGTCGC650.10507937534352872No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTANCCNCGCNTTCGCGCTTCAG650.10507937534352872No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCACGCATTAG650.10507937534352872No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG630.10184616379449707No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTANCCNCGNTTTCGCGCTTCAG630.10184616379449707No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)