FastQCFastQC Report
Tue 8 Aug 2023
EGAF00008120116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008120116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34791
Sequences flagged as poor quality0
Sequence length301
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3571.026127446753471No Hit
GACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3350.9628927021356098No Hit
GACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG3240.9312753298266794No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG3220.9255267166796011No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG3150.9054065706648271No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2990.859417665488201No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2970.8536690523411227No Hit
GACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2660.7645655485614096No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2630.7559426288407922No Hit
GACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2510.7214509499583226No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2400.689833577649392No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2320.666839125061079No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2300.6610905119140007No Hit
GACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG2240.6438446724727659No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG2140.6151016067373746No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG2070.5949814607226007No Hit
GACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1900.5461182489724354No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1880.5403696358253571No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1850.5317467161047397No Hit
GACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1800.5173751832370441No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1720.494380730648731No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG1670.4800091977810353No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1580.4541404386191831No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1580.4541404386191831No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG1540.4426432123250266No Hit
GACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1510.43402029260440916No Hit
GACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG1380.3966543071484005No Hit
GACTACTCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1070.3075508033686873No Hit
GACTACTAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG1050.30180219022160903No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG940.27018481791267857No Hit
GNNTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG850.24431605875082635No Hit
GACTACTGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG820.23569313903020897No Hit
GNNTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG800.2299445258831307No Hit
GACTACCGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG800.2299445258831307No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG770.2213216061625133No Hit
GNNTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG770.2213216061625133No Hit
GACTACAGGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG720.20695007329481763No Hit
GACTACCAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG690.19832715357420025No Hit
GNNTANCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG690.19832715357420025No Hit
GACTACAAGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG680.1954528470006611No Hit
GACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG670.19257854042712194No Hit
GACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG660.18970423385358284No Hit
GNNTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG620.17820700755942628No Hit
GACTACACGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG610.17533270098588716No Hit
GNNTANTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG610.17533270098588716No Hit
GNNTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG600.172458394412348No Hit
GNNTANTCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG600.172458394412348No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG590.1695840878388089No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG590.1695840878388089No Hit
GACTACCCGGGTATCTAATCCTGTTCGCTCCCCATGCTTTCGCTTCTCAG570.16383547469173063No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG570.16383547469173063No Hit
GNNTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG570.16383547469173063No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG570.16383547469173063No Hit
GANTANTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG560.16096116811819147No Hit
NANTNNTCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG550.15808686154465235No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG550.15808686154465235No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG550.15808686154465235No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG550.15808686154465235No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG550.15808686154465235No Hit
NNNTNNTGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG550.15808686154465235No Hit
NANTNNTAGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG540.15521255497111322No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG540.15521255497111322No Hit
GNNTANTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG540.15521255497111322No Hit
GACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG530.15233824839757407No Hit
GANTANTCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG530.15233824839757407No Hit
GNNTANTGGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG530.15233824839757407No Hit
GACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG510.14658963525049581No Hit
GNNTANAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG510.14658963525049581No Hit
NANTNNTAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG500.1437153286769567No Hit
NANTNNCCGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG500.1437153286769567No Hit
GANTANTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG500.1437153286769567No Hit
GANTANTGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG500.1437153286769567No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG500.1437153286769567No Hit
GANTANTCGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG500.1437153286769567No Hit
GANTNNTCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG500.1437153286769567No Hit
GNNTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG480.13796671552987844No Hit
GANTNNTGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG480.13796671552987844No Hit
NNNTNNCGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG470.13509240895633928No Hit
NNCTNNTCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG470.13509240895633928No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG470.13509240895633928No Hit
NANTANCGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG470.13509240895633928No Hit
NANTNNTCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG460.13221810238280016No Hit
GANTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG460.13221810238280016No Hit
NANTNNTGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG460.13221810238280016No Hit
NNNTNNCGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG450.12934379580926103No Hit
NANTANCGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG450.12934379580926103No Hit
NNNTNNTAGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG450.12934379580926103No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG450.12934379580926103No Hit
NNNTNNTAGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG450.12934379580926103No Hit
NANTANTGGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG440.12646948923572188No Hit
GANTANTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG440.12646948923572188No Hit
GNNTANTGGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG440.12646948923572188No Hit
GNNTANCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG440.12646948923572188No Hit
GNNTANCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG430.12359518266218275No Hit
GANTNNTCGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGAGCCNCAG430.12359518266218275No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG420.12072087608864361No Hit
NNCTNNTAGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG410.11784656951510449No Hit
GANTNNTAGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG410.11784656951510449No Hit
GNNTANCCGGGTNTNTNNTNNTGTTTGNTCNCNNCGNTTTCGAGCCTNNG410.11784656951510449No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG410.11784656951510449No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG410.11784656951510449No Hit
NANTNNCCGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG400.11497226294156535No Hit
NNNTNNCCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG400.11497226294156535No Hit
NANTNNCAGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGCTCCTCAG400.11497226294156535No Hit
GNNTANAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG400.11497226294156535No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG400.11497226294156535No Hit
GNNTANACGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG400.11497226294156535No Hit
NANTNNTGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG400.11497226294156535No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGAGCCTCAG400.11497226294156535No Hit
NNNTNNTAGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG390.11209795636802622No Hit
GANTNNCGGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG390.11209795636802622No Hit
GNNTANACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG390.11209795636802622No Hit
NNCTNNTGGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG390.11209795636802622No Hit
GANTANTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG390.11209795636802622No Hit
NANTANTAGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG390.11209795636802622No Hit
GACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG390.11209795636802622No Hit
NANTANTCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGAGCCTCAG380.10922364979448708No Hit
NNNTNNTCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG380.10922364979448708No Hit
GNNTANAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG380.10922364979448708No Hit
NNNTNNTCGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGAGCCNNNG380.10922364979448708No Hit
NNNTNNCGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG380.10922364979448708No Hit
GANTANCGGGGTNTCTNNTNNTGTTTGCTCCCCACGNTTTCGAGCCTCAG380.10922364979448708No Hit
GANTNNCCGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG380.10922364979448708No Hit
NNCTNNTCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGAGCCTCAG380.10922364979448708No Hit
NNNTNNTGGGNNNTNTNATNNTGTTCGNTCNCCNCGNTTTCGCTCCNNNG380.10922364979448708No Hit
GANTNNTGGGGTATNTAATNNTGTTTGNTCNCCNNGCTTTCGAGCCNCAG370.10634934322094795No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGCTCCTCAG370.10634934322094795No Hit
GANTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG370.10634934322094795No Hit
GANTNNCAGGGTATNTAATNNTGTTCGNTCNCCNNGCTTTCGCTCCNCAG370.10634934322094795No Hit
NANTANTCGGGTATNTANTNNTGTTTGNTCNCCNNGNTTTCGAGCCTCAG370.10634934322094795No Hit
NANTANTGGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGAGCCTCAG370.10634934322094795No Hit
NNNTNNTGGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG360.10347503664740881No Hit
GACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG360.10347503664740881No Hit
NNNTNNTGGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGAGCCTCAG360.10347503664740881No Hit
NANTANCCGGGTATNTANTNNTGTTCGNTCNCCNNGNTTTCGCTCCTCAG360.10347503664740881No Hit
GANTANCGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGCTCCTCAG360.10347503664740881No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGAGCCTNNG360.10347503664740881No Hit
NNCTNNCCGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG360.10347503664740881No Hit
NNCTNNCCGGGTATNTANTCNTGTTCGNTCNCCNCGCTTTCGCTCCTCAG360.10347503664740881No Hit
GACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCAG350.10060073007386967No Hit
NNNTNNTCGGGTATNTNNTNNTGTTTGNTCNCCNCGNTTTCGAGCCTCAG350.10060073007386967No Hit
GANTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG350.10060073007386967No Hit
NNNTNNCCGGGTATNTNNTNNTGTTCGNTCNCCNCGNTTTCGCTCCTCAG350.10060073007386967No Hit
GANTANTGGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGAGCCTCAG350.10060073007386967No Hit
NANTNNCGGGGTNTNTNNTCNTGTNTGNTCNCCNCGCNTTCGAGCCTCAG350.10060073007386967No Hit
NNCTNNTGGGGTATNTANTCNTGTTTGNTCNCCNCGCTTTCGAGCCTCAG350.10060073007386967No Hit
GANTANCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCAG350.10060073007386967No Hit
NNNTNNAGGGNNNTNTNATNNTGTTTGNTCNCCNCGNTTTCGAGCCNNNG350.10060073007386967No Hit
GNNTANTCGGGTNTNTNNTNNTGTTCGNTCNCNNCGNTTTCGCTCCTNNG350.10060073007386967No Hit
GANTANTAGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGAGCCTCAG350.10060073007386967No Hit
GANTANCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCAG350.10060073007386967No Hit
NANTNNCGGGGTNTNTNNTCNTGTNCGNTCNCCNCGCNTTCGAGCCTCAG350.10060073007386967No Hit
GANTANCCGGGTNTCTNNTNNTGTTCGCTCCCCACGNTTTCGAGCCTCAG350.10060073007386967No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)